Effect of a statherin-derived peptide (Stn15pSpS) on the prevention of erosive wear of enamel and dentine in vitro

• Grant number: 20/01634-7
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): May 01, 2020
• Effective date (End): December 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Fabiana Navas Reis
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated scholarship(s): 21/09013-4 - Effect of statherin-derived peptide (Stn15pSpS) on the thickness and morphology of the acquired enamel pellicle: evaluation by transmission electron microscopy, BE.EP.MS

Abstract

Due to the multifactorial etiology of dental erosion, there are several preventive and therapeutic possibilities. Saliva is one of the most important protective factors involved, also contributing to the formation of the acquired pellicle. The incorporation of proteins into the acquired film, through its addition to dental products such as rinse solutions or gels, for example, may affect its ability to protect against erosion. Preliminary experiments from our group revealed a peptide derived from staherin cotaning the 15 aminoacids of the N-terminus, with serines 2 and 3 phosphorylated, protects the enamel against initial erosion. However, there are neither studies evaluating this protective potential upon more severe erosive challenges, nor studies evaluating dentin. The objective of the present study is to evaluate the effect of solutions or gels containing Stn15pSpSCaneCPI-5, in different concentrations, in the protection against erosion of enamel and dentin in vitro. 120 blocks of enamel and 144 blocks of bovine dentin will be prepared. Four groups will be formed for solutions [(negative control (placebo, deionized water), positive control (Elmex Erosion Protection, Colgate) and experimental (solutions containing Stn15pSpS at 1.88X10-5 M or 3.76 X10-5 M)] and another 4 groups for the gels [(negative control (placebo gel), positive control (gel containing 12300 ppm F as NaF) and experimental (gels containing Stn15pSpS at 1.88X10-5 M or 3.76 X10-5 M)]. The solutions will be applied for 1 min and the gels for 4 min. Stimulated saliva will be collected from 3 volunteers for the formation of the acquired pellicle (for 2 h) on the specimens. Afterwards, the specimens will undergo an erosive pH cycling 4X / day for 7 days. Each cycle will consist of: immersion of the specimens in 0.1% citric acid pH 2.5 for 90 s, washing in deionized water for 5 s, immersion in artificial saliva for 2 h and washing with deionized water for 5 s. The solutions and gels will be applied during the pH cycling, 2X / day for 1 min and 4 min, respectively, after the first and last erosive challenge. The specimens will be immersed in artificial saliva overnight, completing every 24 h of the cycling. The wear (¼m) will be evaluated by contact profilometry. The data will be analyzed in relation to normality and homogeneity, to select the appropriate statistical test (p <0.05). (AU)