Effect of Statherin-derived peptide (Stn15pSpS) associated or not with epigallocatechin-3-gallate (EGCG) against enamel erosive wear in situ

• Grant number: 23/07157-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: December 01, 2023
• End date: October 15, 2024
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Lethycia Almeida Santos Azuma
• Supervisor: Matthias Hannig
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: Saarland University, Homburg, Germany
• Associated to the scholarship: 21/03497-0 - Engineering of acquired enamel film to protect against erosive dental wear: evaluation of the protective effect of cystatin derived from sugarcane (CaneCPI-5), hemoglobin and/or peptide derived from staterine, associated or not with EGCG, BP.DR

Abstract

Acquired enamel pellicle (AEP) engineering, by immobilizing molecules with high binding capacity to hydroxyapatite and resistance to removal by acids, is a new strategy for controlling erosive tooth wear (ETW). Our group has been employing a statherin-derived peptide (StN15pSpS) in strategies of this type. Polyphenols, such as epigallocatechin-3-gallate (EGCG), are readily adsorbed to AEP, making it rougher, thicker and more electron-dense, increasing the precipitation of protective proteins in the AEP, such as albumin, statherin and cystatins. Therefore, in this project we aim to answer the following question: Does the rinse with EGCG associated with Stn15pSpS increase enamel resistance to ETW, in comparison with rinse with the peptide alone? AEP will be formed by 3 volunteers on bovine enamel specimens fixed to individual acrylic splints. After intraoral exposure of the enamel specimens for 3 minutes, the volunteer will rinse for 1 min with 10 ml of the treatment solutions: statherin solution (1.88 X 10-5 M) or statherin with EGCG (0.46 mg/mL). After 3 min and 120 min, enamel samples will be collected and covered with one drop (8 µl) of 1 % citric acid (pH 2.5) or 0.01 M HCl (pH 2.0) for 10 s. The ultrastructure of the pellicle will be examined by Transmission Electron Microscopy. Additionally, the immunodetection will be performed by Fusion SL image acquisition system. (AU)