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Engineering of acquired enamel film to protect against erosive dental wear: evaluation of the protective effect of cystatin derived from sugarcane (CaneCPI-5), hemoglobin and/or peptide derived from staterine, associated or not with EGCG

Grant number: 21/03497-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2022
Effective date (End): October 31, 2025
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Marília Afonso Rabelo Buzalaf
Grantee:Lethycia Almeida Santos Azuma
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated research grant:19/26070-1 - Modulation of acquired enamel pellicle to control dental mineral loss: unravelling mechanisms to make therapies possible, AP.TEM
Associated scholarship(s):23/07157-4 - Effect of Statherin-derived peptide (Stn15pSpS) associated or not with epigallocatechin-3-gallate (EGCG) against enamel erosive wear in situ, BE.EP.DR


The engineering of acquired enamel film (PAE), due to the immobilization of molecules with high binding capacity to hydroxyapatite and resistance to acid removal, is a new strategy for the control of erosive tooth wear (DDE). Our group has been using cystatin derived from sugar cane (CaneCPI-5), a peptide derived from staterin (StN15) and human hemoglobin (Hb) in strategies of this type. Polyphenols, such as EGCG, readily adsorb to PAE, making it more rough, thick and electron-dense, increasing the precipitation of protective proteins in PAE, such as albumin, staterin and cystatines. Therefore, in this project we intend to answer the following question: The treatment of the dental surface with EGCG associated with CaneCPI-5, Stn15pSpS or Hb, isolated or combined, alters the free surface energy, increases the incorporation of acid-resistant proteins to PAE and the resistance of enamel to erosive demineralization, compared to treatment with proteins only? To answer this question, 2 studies will be carried out, one in vivo and the other in vitro. In the in vitro study, volunteers will rinse with the solutions containing the isolated or combined proteins, the PAE will be formed for 3 min, later it will be rinsed with EGCG or not and the PAE will have its formation completed for 120 min. After this time, an erosive challenge will be made with citric acid, a drop of the acid will be collected for calcium analysis and the acid-resistant proteins in the PAE will be collected for proteomic analysis. In the in vitro experiment, the free surface energy after these treatments will be evaluated by the sessile drop method, using a goniometer. (AU)

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