The profile secretion of Aspergillus clavatus: Different pre-treatments of sugarcane bagasse distinctly induces holocellulases for the lignocellulosic biomass conversion into sugar

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de Lucas, Rosymar Coutinho ; de Oliveira, Tassio Brito ; Lima, Matheus Sanita ; Pasin, Thiago Machado ; de Almeida Scarcella, Ana Silvia ; Costa Ribeiro, Liliane Fraga ; Carvalho, Caio ; de Lima Damasio, Andre Ricardo ; Buckeridge, Marcos Silveira ; Prade, Rolf Alexander ; Segato, Fernando ; Teixeira de Moraes Polizeli, Maria de Lourdes

• Document type: Journal article
• Source: RENEWABLE ENERGY; v. 165, p. 10-pg., 2021-03-01.

Abstract

The plant cell wall is the most abundant carbon reservoir in nature and is a renewable source of biofuels. To break down this biomass and convert it into fermentable sugars, a set of multiple enzymes is needed. Here, we characterize the enzymatic repertoire necessary for the degradation of sugarcane bagasse "in natura" and pre-treated using Aspergillus clavatus as a model. 135 unique peptides were identified by Mass Spectrometry MS/MS. 23 of these proteins belong to classes of enzymes involved in biomass degradation and were differentially expressed on various substrates. Each pretreatment changed the sugarcane bagasse composition, which, in turn, led to the differential expression of A. clavatus holocellulases. The deconstruction of "in natura" bagasse demanded the largest set of enzymes due to the structural complexity of this material. Not only different sources of biomass but also different pretreatments of the same source will determine the enzymes required for the most efficient biomass conversion, avoiding the use of non-essential enzymes and consequent financial expense. Understanding A. clavatus nutritional strategies by proteomic analysis of secretome can improve the technology applied to biomass conversion and by-product synthesis. (C) 2020 Published by Elsevier Ltd. (AU)

• FAPESP's process: 17/09000-4 - Effects of temperature and water stress, in climate change scenario, on the fungal community in tropical soil and selection of nitrous oxide producing strains
• Grantee: Tássio Brito de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/02169-7 - Aspergillus nidulans as a model for heterologous expression of cellulases and hemicellulases
• Grantee: André Ricardo de Lima Damasio
• Support Opportunities: Scholarships in Brazil - Post-Doctoral