In-Tube Solid-Phase Microextraction Directly Coupled to Mass Spectrometric Systems: A Review

Author(s)

Grecco, Caroline Fernandes ; de Souza, Israel Donizeti ; Oliveira, Igor Gustavo Carvalho ; Queiroz, Maria Eugenia Costa

• Document type: Journal article
• Source: SEPARATIONS; v. 9, n. 12, p. 19-pg., 2022-12-01.

Abstract

Since it was introduced in 1997, in-tube solid-phase microextraction (in-tube SPME), which uses a capillary column as extraction device, has been continuously developed as online microextraction coupled to LC systems (in-tube SPME-LC). In the last decade, new couplings have been evaluated on the basis of state-of-the-art LC instruments, including direct coupling of in-tube SPME to MS/MS systems, without chromatographic separation, for high-throughput analysis. In-tube SPME coupling to MS/MS has been possible thanks to the selectivity of capillary column coatings and MS/MS systems (SRM mode). Different types of capillary columns (wall-coated open-tubular, porous-layer open-tubular, sorbent-packed, porous monolithic rods, or fiber-packed) with selective stationary phases have been developed to increase the sorption capacity and selectivity of in-tube SPME. This review focuses on the in-tube SPME principle, extraction configurations, current advances in direct coupling to MS/MS systems, experimental parameters, coatings, and applications in different areas (food, biological, clinical, and environmental areas) over the last years. (AU)

• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/19485-0 - Development of nitinol fiber chemically coated with zwitterionic polymeric ionic liquids for determination of beta-amyloids in plasma samples from patients with Alzheimer's disease by direct coupling fiber-in-tube SPME and MS/MS
• Grantee: Israel Donizeti de Souza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/00126-8 - Development of the methods of the microextraction hyphenated with LC-MS/MS or directly to MS/MS for the determination of biomarkers in biological samples of patient or rats with neurodegenerative diseases
• Grantee: Maria Eugênia Queiroz Nassur
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/06526-8 - Development of an organic monolithic phase with anion exchanger groups and hydrophilic coating to homocysteine and homocysteic acid determination in biological samples from patients with Alzheimer's Disease by in-tube SPME-UHPLC-MS/MS
• Grantee: Igor Gustavo de Carvalho Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/04386-7 - Development of an organic monolithic phase for the determination of b-amyloid peptides AB40 and AB42 in plasma samples from patients with Alzheimer's Disease by UHPLC-MS/MS in column switching mode
• Grantee: Caroline Fernandes Grecco
• Support Opportunities: Scholarships in Brazil - Doctorate