Development of an HPLC Method for Identification and Quantification of Anti-leishmaniasis Drug Candidate NFOH After Oral Administration of NLC-NFOH in Rats

Author(s)

de Souza, Aline ; Lima de Almeida, Maria Karilly ; Barbosa, Joao Antonio ; Yukuyama, Megumi Nishitani ; Correia, Cristiano Jesus ; Breithaupt-Faloppa, Ana Cristina ; Bou-Chacra, Nadia Araci ; Calixto, Leandro Augusto

• Document type: Journal article
• Source: CHEMISTRY AFRICA-A JOURNAL OF THE TUNISIAN CHEMICAL SOCIETY; v. N/A, p. 7-pg., 2022-11-28.

Abstract

Hydroxymethylnitrofurazone (NFOH) is a prodrug synthesized from the original nitrofurazone compound (NF). NFOH has shown activity in vivo against Trypanosoma cruzi and in vitro activity against Leishmania amazonensis. However, it has shown less toxicity than NF. This study aims to develop and evaluate the selectivity, carryover, matrix effect, linearity, precision and accuracy, of a bioanalytical method using HPLC to quantify NFOH and NF. The defined method conditions were mobile phase acetonitrile: water (20:80 v/v), Zorbax SB-C18, 5 mu m (4.6 x 250 mm) flow rate of 1.2 mL min(-1), at UV detection of 370 nm. The linearity obtained for NFOH and NF was in the range of 0.025-3.0 mu g mL(-1) with a correlation coefficient > 0.98. The precision (relative standard deviation) was 2.44-13.77% and 2.61-18.42% for NFOH and NF, respectively; the accuracy was 2.66-14.28% and 2.09-19.06% for NFOH and NF, respectively. In conclusion this method was successfully developed to determine and quantify NFOH and NF in serum after oral administration of nanostructured lipid carrier with NFOH. It opens opportunities to be applied in in vivo studies in novel NFOH nanoparticles formulations. (AU)

• FAPESP's process: 17/08332-3 - NANOSTRUCTURED SYSTEMS WITH POTENTIAL APPLICATION IN TREATMENT OF NEGLECTED DISEASES
• Grantee: Nádia Araci Bou-Chacra
• Support Opportunities: Regular Research Grants