Proteogenomics identification of TBBPA degraders in anaerobic bioreactor

Author(s)

Macedo, Williane Vieira ; Poulsen, Jan Struckmann ; Zaiat, Marcelo ; Nielsen, Jeppe Lund

• Document type: Journal article
• Source: Environmental Pollution; v. 310, p. 9-pg., 2022-08-02.

Abstract

Tetrabromobisphenol A (TBBPA) is the most used flame retardant worldwide and has become a threat to aquatic ecosystems. Previous research into the degradation of this micropollutant in anaerobic bioreactors has suggested several identities of putative TBBPA degraders. However, the organisms actively degrading TBBPA under in situ conditions have so far not been identified. Protein-stable isotope probing (protein-SIP) has become a cutting-edge technique in microbial ecology for enabling the link between identity and function under in situ conditions. Therefore, it was hypothesized that combining protein-based stable isotope probing with metagenomics could be used to identify and provide genomic insight into the TBBPA-degrading organisms. The identified 13C-labelled peptides were found to belong to organisms affiliated to Phytobacter, Clostridium, Sporolactobacillus, and Klebsilla genera. The functional classification of identified labelled peptides revealed that TBBPA is not only transformed by cometabolic reactions, but also assimilated into the biomass. By application of the proteogenomics with labelled micropollutants (protein-SIP) and metagenome-assembled genomes, it was possible to extend the cur-rent perspective of the diversity of TBBPA degraders in wastewater and predict putative TBBPA degradation pathways. The study provides a link to the active TBBPA degraders and which organisms to favor for optimized biodegradation. (AU)

• FAPESP's process: 20/11984-5 - Tebrabromobisphenol A (TBBPA) biodegradation by complex microbial communities: uncovering metabolic pathways by meta-omics analysis
• Grantee: Williane Vieira Macêdo
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/17744-6 - Combined system for the treatment of Tetrabromobisphenol a (TBBPA) by anaerobic digestion and advanced oxidative processes
• Grantee: Williane Vieira Macêdo
• Support Opportunities: Scholarships in Brazil - Doctorate