Investigation of U-251 cell death triggered by flavonoid luteolin: towards a better understanding on its anticancer property against glioblastomas

Author(s)

Franco, Yollanda E. M. ; de Lima, Carolina A. ; Rosa, Marcela N. ; Silva, Viviane A. O. ; Reis, Rui M. ; Priolli, Denise G. ; Carvalho, Patricia O. ; do Nascimento, Jessyane R. ; da Rocha, Claudia Q. ; Longato, Giovanna B.

• Document type: Journal article
• Source: NATURAL PRODUCT RESEARCH; v. 35, n. 22, p. 7-pg., 2020-02-20.

Abstract

Recently, many studies have reported the anticancer properties of flavonoid luteolin against a variety of tumors, but there is still a lack in the description of its mechanism of action. In attempt to better contribute to the literature, we evaluated the antiproliferative activity of luteolin extracted by Fridericia platyphylla in a panel of tumor cell lines representative of six different tissues. Luteolin presented antiproliferative activity for all the assessed tumor cell lines, being glioblastoma the most sensitive one. This compound was able to inhibit U-251 cells migration and tumorigenesis. Besides, luteolin leads U-251 tumor cells to apoptosis death by depolarisation of the mitochondrial membrane, ERK proteins phosphorylation, cleavage of PARP and Caspase 9, further inducing DNA damage by H2AX phosphorylation, which had not yet been described for glioblastomas. Altogether, our results reaffirm luteolin as a potential therapeutic drug. (AU)

• FAPESP's process: 16/06137-6 - Bioprospecting for natural compounds and bioactive derivatives with potent anticancer activity: in vitro/in vivo assays and investigation into the mechanism of action.
• Grantee: Giovanna Barbarini Longato
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/02345-3 - In vitro and in vivo study of the anticancer activity of flavonoids and biotransformed flavonoids
• Grantee: Yollanda Edwirges Moreira Franco
• Support Opportunities: Scholarships in Brazil - Master