| Full text | |
| Author(s): |
Barboza, Bruno Rafael
;
Thomaz, Sandra Maria de Oliveira
;
de Carvalho, Airton
;
Espreafico, Enilza Maria
;
Miyamoto, Jackson Gabriel
;
Tashima, Alexandre Keiji
;
Camacho, Mauricio Frota
;
Zelanis, Andre
;
Roque-Barreira, Maria Cristina
;
da Silva, Thiago Aparecido
Total Authors: 10
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 2, p. 24-pg., 2023-01-01. |
| Abstract | |
Receptors on the immune cell surface have a variety of glycans that may account for the immunomodulation induced by lectins, which have a carbohydrate recognition domain (CRD) that binds to monosaccharides or oligosaccharides in a specific manner. ArtinM, a D-mannose-binding lectin obtained from Artocarpus heterophyllus, has affinity for the N-glycans core. Immunomodulation by ArtinM toward the Th1 phenotype occurs via its interaction with TLR2/CD14 N-glycans on antigen-presenting cells, as well as recognition of CD3 gamma N-glycans on murine CD4+ and CD8+ T cells. ArtinM exerts a cytotoxic effect on Jurkat human leukemic T-cell line and human myeloid leukemia cell line (NB4). The current study evaluated the effects of ArtinM on murine and human B cells derived from non-Hodgkin's lymphoma. We found that murine B cells are recognized by ArtinM via the CRD, and the ArtinM stimulus did not augment the proliferation rate or production of IL-2. However, murine B cell incubation with ArtinM augmented the rate of apoptosis, and this cytotoxic effect of ArtinM was also seen in human B cell-lines sourced from non-Hodgkin's lymphoma Raji cell line. This cytotoxic effect was inhibited by the phosphatase activity of CD45 on Lck, and the protein kinases of the Src family contribute to cell death triggered by ArtinM. (AU) | |
| FAPESP's process: | 16/04877-2 - Design of new therapeutic strategies, based on the carbohydrate recognition, against cryptococcosis |
| Grantee: | Thiago Aparecido da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/18538-0 - Bioengineered of T- and NK-cells by CAR against invasive fungal infections |
| Grantee: | Thiago Aparecido da Silva |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 18/21708-5 - Application of immunomoduladors, by carbohydrate recognition, as therapeutic agents: mechanism of action to immunotherapy |
| Grantee: | Maria Cristina Roque Antunes Barreira |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/05867-9 - Effect of ArtinM on B and NK cells |
| Grantee: | Bruno Rafael Barboza |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 17/20106-9 - Peptidomics of Brazilian snake and spider venoms |
| Grantee: | Alexandre Keiji Tashima |
| Support Opportunities: | Regular Research Grants |