| Full text | |
| Author(s): |
Bernardo, Desiree Rita Denelle
;
Canale, Daniele
;
Nascimento, Mariana Moura
;
Shimizu, Maria Heloisa Massola
;
Seguro, Antonio Carlos
;
de Braganca, Ana Carolina
;
Volpini, Rildo Aparecido
Total Authors: 7
|
| Document type: | Journal article |
| Source: | FRONTIERS IN NUTRITION; v. 9, p. 22-pg., 2022-11-17. |
| Abstract | |
Acute kidney injury (AKI) alters renal hemodynamics, leading to tubular injury, activating pathways of inflammation, proliferation, and cell death. The initial damage caused to renal tissue after an ischemia/reperfusion (I/R) injury exerts an important role in the pathogenesis of the course of AKI, as well as in the predisposition to chronic kidney disease. Vitamin D deficiency has been considered a risk factor for kidney disease and it is associated with tubulointerstitial damage, contributing to the progression of kidney disease. Obesity is directly related to diabetes mellitus and hypertension, the main metabolic disorders responsible for the progression of kidney disease. Furthermore, the expansion of adipose tissue is described as an important factor for increased secretion of pro-inflammatory cytokines and their respective influence on the progression of kidney disease. We aimed to investigate the influence of vitamin D deficiency and obesity on the progression of renal disease in a murine model of renal I/R. Male Wistar rats underwent renal I/R surgery on day 45 and followed until day 90 of the protocol. We allocated the animals to four groups according to each diet received: standard (SD), vitamin D-depleted (VDD), high fat (HFD), or high fat vitamin D-depleted (HFDV). At the end of 90 days, we observed almost undetectable levels of vitamin D in the VDD and HFDV groups. In addition, HFD and HFDV groups presented alterations in the anthropometric and metabolic profile. The combination of vitamin D deficiency and obesity contributed to alterations of functional and hemodynamic parameters observed in the HFDV group. Moreover, this combination favored the exacerbation of the inflammatory process and the renal expression of extracellular matrix proteins and phenotypic alteration markers, resulting in an enlargement of the tubulointerstitial compartment. All these changes were associated with an increased renal expression of transforming growth factor beta and reduced expression of the vitamin D receptor. Our results show that the synergistic effect of obesity and vitamin D deficiency exacerbated the hemodynamic and morphological changes present in the evolution of renal disease induced by I/R. (AU) | |
| FAPESP's process: | 18/12297-1 - Analysis of alternative events involved in the formation of renal fibrosis in vitamin D deficient rats submitted to nephrectomy 5/6 |
| Grantee: | Rildo Aparecido Volpini |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/20840-0 - Evaluation of the therapeutic effects of Nebivolol on TDF-induced nephrotoxicity in rats |
| Grantee: | Daniele Canale Cavicchioli |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/04930-6 - Study of vitamin D replacement in the evolution of ischemic acute kidney injury in vitamin D deficient rats |
| Grantee: | Ana Carolina de Bragança Viciana |
| Support Opportunities: | Regular Research Grants |