MicroRNA-149-3p expression correlates with outcomes of adrenocortical tumor patients and affects proliferation and cell cycle progression of H295A adrenocortical cancer cell line

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da Silva, Keteryne Rodrigues ; Veronez, Luciana Chain ; Pereira Correa, Carolina Alves ; Peixoto Lira, Regia Caroline ; Baroni, Mirella ; Silva Queiroz, Rosane de Paula ; Rauber Antonini, Sonir Roberto ; Yunes, Jose Andres ; Brandalise, Silvia Regina ; Tone, Luiz Gonzaga ; Scrideli, Carlos Alberto

• Document type: Journal article
• Source: HUMAN CELL; v. 35, n. 6, p. 9-pg., 2022-09-02.

Abstract

Pediatric adrenocortical tumor (ACT) is a rare and aggressive neoplasm, with incidence in southern and southeastern Brazil 10-15 times higher than worldwide. Although microRNAs (miRNAs) have been reported to act as tumor suppressors or oncogenes in several cancers, the role of miR-149-3p in ACT remains unknown. In this study, we evaluated the expression of miR-149-3p in 67 pediatric ACT samples and 19 non-neoplastic adrenal tissues. The overexpression of miR-149-3p was induced in H295A cell line, and cell viability, proliferation, colony formation, and cell cycle were assessed by in miR-149-3p mimic or mimic control. In silico analysis were used to predict miR-149-3p putative target genes. CDKN1A expression at the mRNA and protein levels was evaluated by qRT-PCR and western blot, respectively. Higher miR-149-3p expression was associated with unfavorable ACT outcomes. Compared to the mimic control, miR-149-3p overexpression increased cell viability and colony formation, and affected cell cycle progression. Also, we identified CDKN1A as a potential miR-149-3p target gene, with decreased expression at both the gene and protein levels in miR-149-3p mimic cells. Collectively, these findings suggest that miR-149-3p promotes H295A cell viability by downregulating CDKN1A and provide evidence that miR-149-3p may be useful as a novel therapeutic target for pediatric ACT (AU)

• FAPESP's process: 10/07020-9 - Molecular studies in neoplasias of children and adolescents: microRNAs signature and functional studies of candidate genes to target therapy
• Grantee: Carlos Alberto Scrideli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/20341-0 - Interactions between emerging therapeutic targets and developmental pathways associated with tumorigenesis: emphasis on pediatric malignancies
• Grantee: Luiz Gonzaga Tone
• Support Opportunities: Research Projects - Thematic Grants