Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation

Author(s)

Vieira, Willians Fernando ; Malange, Kaue Franco ; de Magalhaes, Silviane Fernandes ; Paes Lemes, Julia Borges ; dos Santos, Gilson Goncalves ; Nishijima, Catarine Massucato ; Rodrigues de Oliveira, Alexandre Leite ; da Cruz-Hofling, Maria Alice ; Parada, Carlos Amilcar ; Tambeli, Claudia Herrera

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 12, n. 1, p. 18-pg., 2022-10-06.

Abstract

Several recent studies have established the efficacy of photobiomodulation therapy (PBMT) in painful clinical conditions. Diabetic neuropathy (DN) can be related to activating mitogen-activated protein kinases (MAPK), such as p38, in the peripheral nerve. MAPK pathway is activated in response to extracellular stimuli, including interleukins TNF-alpha and IL-1 beta. We verified the pain relief potential of PBMT in streptozotocin (STZ)-induced diabetic neuropathic rats and its influence on the MAPK pathway regulation and calcium (Ca2+) dynamics. We then observed that PBMT applied to the L4-L5 dorsal root ganglion (DRG) region reduced the intensity of hyperalgesia, decreased TNF-alpha and IL-1 beta levels, and p38-MAPK mRNA expression in DRG of diabetic neuropathic rats. DN induced the activation of phosphorylated p38 (p-38) MAPK co-localized with TRPV1(+) neurons; PBMT partially prevented p-38 activation. DN was related to an increase of p38-MAPK expression due to proinflammatory interleukins, and the PBMT (904 nm) treatment counteracted this condition. Also, the sensitization of DRG neurons by the hyperglycemic condition demonstrated during the Ca2+ dynamics was reduced by PBMT, contributing to its anti-hyperalgesic effects. (AU)

• FAPESP's process: 18/05108-8 - Direct influence of low-level laser irradiation on neurons: an electrophysiological study to helping comprise analgesic effects of infrared light in diabetic neuropathy
• Grantee: Willians Fernando Vieira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 15/12673-5 - Effects of low-level laser therapy (LLLT) in experimental diabetic neuropathy
• Grantee: Willians Fernando Vieira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/25153-7 - Analysis of differential gene expression of dorsal ganglion root cell in a model of diabetes and peripheral diabetic neuropathy
• Grantee: Carlos Amilcar Parada
• Support Opportunities: Regular Research Grants