The fibrotic niche impairs satellite cell function and muscle regeneration in mouse models of Marfan syndrome

Author(s)

da Silva, Meiricris Tomaz ; Santos, Audrei R. ; Koike, Tatiana E. ; Nascimento, Tabata L. ; Rozanski, Andrei ; Bosnakovski, Darko ; Pereira, Lygia, V ; Kumar, Ashok ; Kyba, Michael ; Miyabara, Elen H.

• Document type: Journal article
• Source: ACTA PHYSIOLOGICA; v. 237, n. 1, p. 19-pg., 2022-10-17.

Abstract

Aim It has been suggested that the proliferation and early differentiation of myoblasts are impaired in Marfan syndrome (MFS) mice during muscle regeneration. However, the underlying cellular and molecular mechanisms remain poorly understood. Here, we investigated muscle regeneration in MFS mouse models by analyzing the influence of the fibrotic niche on satellite cell function. Methods In vivo, ex vivo, and in vitro experiments were performed. In addition, we evaluated the effect of the pharmacological inhibition of fibrosis using Ang-(1-7) on regenerating skeletal muscles of MFS mice. Results The skeletal muscle of MFS mice shows an increased accumulation of collagen fibers (81.2%), number of fibroblasts (157.1%), and Smad2/3 signaling (110.5%), as well as an aberrant number of fibro-adipogenic progenitor cells in response to injury compared with wild-type mice. There was an increased number of proinflammatory and anti-inflammatory macrophages (3.6- and 3.1-fold, respectively) in regenerating muscles of wild-type mice, but not in the regenerating muscles of MFS mice. Our data show that proliferation and differentiation of satellite cells are altered (p <= 0.05) in MFS mice. Myoblast transplantation assay revealed that the regenerating muscles from MFS mice have reduced satellite cell self-renewal capacity (74.7%). In addition, we found that treatment with Ang-(1-7) reduces fibrosis (71.6%) and ameliorates satellite cell dysfunction (p <= 0.05) and muscle contractile function (p <= 0.05) in MFS mice. Conclusion The fibrotic niche, caused by Fbn1 mutations, reduces the myogenic potential of satellite cells, affecting structural and functional muscle regeneration. In addition, the fibrosis inhibitor Ang-(1-7) partially counteracts satellite cell abnormalities and restores myofiber size and contractile force in regenerating muscles. (AU)

• FAPESP's process: 17/14115-5 - Study of the satellite cell regenerative capacity in mice with Marfan syndrome
• Grantee: Meiricris Tomaz da Silva
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 20/15351-7 - Role of beta 2 adrenoceptor in satellite cell proliferation and differentiation
• Grantee: Elen Haruka Miyabara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/24946-4 - Rehabilitation of skeletal muscle of mice with Marfan syndrome: effect of eccentric exercise
• Grantee: Elen Haruka Miyabara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/09069-4 - The influence of beta 2-adrenoceptor in satellite cell function
• Grantee: Tatiana Emy Koike
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/23391-8 - Regenerative response of skeletal muscles from mice with Marfan Syndrome
• Grantee: Elen Haruka Miyabara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/04783-0 - The influence of heat shock proteins in the recovery of skeletal muscle of old mice
• Grantee: Tábata Leal Nascimento
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/13874-1 - Skeletal muscle regeneration in mice with Marfan Syndrome: study of the regenerative potential of satellite cells
• Grantee: Meiricris Tomaz da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral