| Full text | |
| Author(s): Show less - |
dos Santos, Laura M. S.
;
de Oliveira, Jonathas M.
;
da Silva, Elaine C. O.
;
Fonseca, Vitor M. L.
;
Silva, Juliane P.
;
Barreto, Emiliano
;
Dantas, Noelio Oliveira
;
Silva, Anielle C. A.
;
Jesus-Silva, Alcenisio J.
;
Mendonca, Cleber R.
;
Fonseca, Eduardo J. S.
Total Authors: 11
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| Document type: | Journal article |
| Source: | Journal of Biomedical Materials Research Part A; v. 111, n. 2, p. 11-pg., 2022-10-14. |
| Abstract | |
Microgrooved surfaces are recognized as an important strategy of tissue engineering to promote the alignment of bone cells. In this work, we have investigated the mechanical and morphological aspects of osteoblasts cells after interaction with different micro-structured polymeric surfaces. Femtosecond laser writing technique was used for the construction of circular and parallel microgrooved patterns in biocompatible polymeric surfaces based on pentaerythritol triacrylate. Additionally, we have studied the influence of the biocompatible TiO2 nanocrystals (NCs) related to the cell behavior, when incorporated to the photoresin. The atomic force microscopy technique was used to investigate the biomechanical reaction of the human osteoblast-like MG-63 cells for the different microgroove. It was demonstrated that osteoblasts grown on circular microgrooved surfaces exhibited significantly larger Young's modulus compared to cells sown on flat films. Furthermore, we could observe that TiO2 NCs improved the circular microgrooves effects, resulting in more populated sites, 34% more elongated cells, and increasing the cell stiffness by almost 160%. These results can guide the design and construction of effective scaffold surfaces with circular microgrooves for tissue engineering and bone regeneration. (AU) | |
| FAPESP's process: | 19/25164-2 - Manufacturing three-dimensional cell scaffolds by two-photon polymerization: strategies for the development of new therapies |
| Grantee: | Cleber Renato Mendonça |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/11283-7 - Nonlinear photonics: spectroscopy and advanced processing of materials |
| Grantee: | Cleber Renato Mendonça |
| Support Opportunities: | Research Projects - Thematic Grants |