Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma

Author(s)

Vieira, Paula ; Jesus, Viviane ; Candido, Marcela Aparecida ; Pacheco-Soares, Cristina ; Castilho, Maiara ; Raniero, Leandro

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 39, p. 7-pg., 2022-07-27.

Abstract

Background: Epidermal Growth Factor Receptor (EGFR receptor) is encoded by the EGFR gene. EGFR receptor signaling pathways are activated by EGF protein, regulating cell actions. Overexpression of EGFR receptor may be linked to malignancies with a poor prognosis. As a result, EGFR receptor is being studied for a variety of tumor diagnostics, spurring the development of innovative approaches to increase quality and efficiency. Nano -materials can recognize cancer cells by specifically targeting of molecular pathways, underscoring the impor-tance of nanomedicine. In this study, we synthesized EGFR-specific nanomarkers by functionalizing EGF protein and Chlorin e6 in gold nanoparticles. These nanoparticles use active targeting to deliver EGF protein to EGFR receptor, and Chlorin e6 serves as a fluorescent marker moleculeMethods: Nanomarkers were examined in vitro in MDA-MB-468 and M059J cell lines. Confocal microscopy and flow cytometry were used to examine the distribution, uptake, internalization, and fluorescence intensity of nanomarkers in vitroResults: The results show that both lines examined accumulate nanomarkers. However, MDA-MB-468 had the highest intensity due to its EGFR receptor overexpression propertiesConclusion: The findings point to ideal properties for detecting EGFR receptor overexpressed cells. (AU)

• FAPESP's process: 17/07519-2 - TREATMENT OF BREAST CANCER IN ANIMAL MODEL VIA PHOTODYNAMIC THERAPY ASSOCIATED WITH BIFUNCTIONAL NANOprobes
• Grantee: Leandro José Raniero
• Support Opportunities: Regular Research Grants