Chemodiversity and Anti-Leukemia Effect of Metabolites from Penicillium setosum CMLD 18

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de Carvalho, Ana Calheiros ; Lima, Caue Santos ; Torquato, Heron Fernandes Vieira ; Domiciano, Andre Tarsis ; Silva, Sebastiao da Cruz ; de Abreu, Lucas Magalhaes ; Uemi, Miriam ; Paredes-Gamero, Edgar Julian ; Vieira, Paulo Cezar ; Veiga, Thiago Andre Moura ; de Medeiros, Livia Soman

• Document type: Journal article
• Source: METABOLITES; v. 13, n. 1, p. 19-pg., 2023-01-01.

Abstract

Penicillium setosum represents a Penicillium species recently described, with little up-to-date information about its metabolic and biological potential. Due to this scenario, we performed chemical and biological studies of P. setosum CMLD18, a strain isolated from Swinglea glutinosa (Rutaceae). HRMS-MS guided dereplication strategies and anti-leukemia assays conducted the isolation and characterization of six compounds after several chromatographic procedures: 2-chloroemodic acid (2), 2-chloro-1,3,8-trihydroxy-6- (hydroxymethyl)-anthraquinone (7), 7-chloroemodin (8), bisdethiobis(methylthio)acetylaranotine (9), fellutanine C (10), and 4-methyl-5,6-diihydro-2H-pyran-2-one (15). From the assayed metabolites, (10) induced cellular death against Kasumi-1, a human leukemia cell line, as well as good selectivity for it, displaying promising cytotoxic activity. Here, the correct NMR signal assignments for (9) are also described. Therefore, this work highlights more detailed knowledge about the P. setosum chemical profile as well as its biological potential, offering prospects for obtaining natural products with anti-leukemia capabilities. (AU)

• FAPESP's process: 18/04095-0 - Analytical Approaches for Discovering Unknown Cytotoxic Compounds from Plants
• Grantee: Thiago André Moura Veiga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/08270-0 - Metabolic diversification of Penicillium fungi isolated from the Atlantic Forest and Amazon biomes: a perspective for biomolecules production
• Grantee: Lívia Soman de Medeiros
• Support Opportunities: Regular Research Grants