Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture

Author(s)

Nogueira, Lucas Fabricio Bahia ; Eufrasio Cruz, Marcos Antonio ; Aguilar, Guilherme Jose ; Tapia-Blacido, Delia Rita ; da Silva Ferreira, Marcia Eliana ; Maniglia, Bianca Chieregato ; Bottini, Massimo ; Ciancaglini, Pietro ; Ramos, Ana Paula

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 13, p. 22-pg., 2022-07-01.

Abstract

Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition. The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2). The biopolymeric membranes were based on hydrolyzed collagen (HC) and chitosan (Cht) or kappa-carrageenan (kappa-carr). The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties. The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1. HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes were bioactive. Hybrid membranes containing Cht were not toxic to the osteoblasts. Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms. Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties. (AU)

• FAPESP's process: 18/25871-8 - Scaffolds composed by bioactive glasses and injectable matrices for bone regeneration
• Grantee: Lucas Fabrício Bahia Nogueira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/08727-0 - Starch modification by green methods for elaboration via 3D printing of bone scaffolds activated by the presence of hydroxyapatite nanoparticles replaced by Sr2+
• Grantee: Bianca Chieregato Maniglia
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/25955-1 - Synthesis and characterization of hybrid biopolymer membranes containing apatites and silver nanoparticles
• Grantee: Lucas Fabrício Bahia Nogueira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/25054-2 - Strontium-containing nanoparticles and their versatility for biomaterials fabrication: implications and applications in biomineralization
• Grantee: Ana Paula Ramos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/05947-2 - Starch modification by green methods for elaboration via 3D printing of bone scaffolds activated by the presence of hydroxyapatite nanoparticles replaced by Sr2+
• Grantee: Bianca Chieregato Maniglia
• Support Opportunities: Scholarships in Brazil - Young Researchers