Starch modification by green methods for elaboration via 3D printing of bone scaffolds activated by the presence of hydroxyapatite nanoparticles replaced by Sr2+

• Grant number: 20/08727-0
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: June 01, 2021
• End date: May 31, 2026
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Bianca Chieregato Maniglia
• Grantee: Bianca Chieregato Maniglia
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated researchers: Ana Paula Ramos
• Associated research grant(s): 24/02072-3 - Printed bone scaffolds based on activated gelatin/chitosan hydrogels by incorporating annatto extract emulsion (Bixa orellana L.), AP.R
• Associated scholarship(s): 25/00927-4 - Development of potential bone scaffolds via 3D printing using hydrogels of microwave-modified starch and surface treatment with cold plasma, BP.MS; 24/20208-0 - 3D printing of bone scaffolds from starch hydrogels activated with plant extracts obtained via natural deep eutectic solvents: a sustainable approach for processability and functionality, BP.MS; 24/16169-9 - "Effect of ozone and dry heating and moist heating technologies on the properties of starches", BP.TT; + associated scholarships 24/13627-6 - Bone scaffolds based on starch hydrogels activated by the presence of nanoparticles of hydroxyapatite functionalized with cobalt and gallium osteinductive ions, BP.IC; 23/17512-6 - Development via 3D printing of bone scaffolds based on starch hydrogels added with annatto extract; investigation of strategies with natural deep eutectic solvents (NADES) and encapsulation, BP.DR; 23/09876-8 - 3D printing of bone scaffolds based on ozone-modified starch gels and added hydroxyapatite nanoparticles, BP.IC; 23/03995-5 - Production via 3D printing and characterization of bioactive bone scaffolds based on starches modified by green technologies and added strontium ions, BP.DD; 22/01565-0 - Study of the 3D printing parameters, BP.TT; 21/08359-4 - Starch modification by green methods for elaboration via 3D printing of bone scaffolds activated by the presence of hydroxyapatite nanoparticles replaced by Sr2+, BP.IC; 21/05947-2 - Starch modification by green methods for elaboration via 3D printing of bone scaffolds activated by the presence of hydroxyapatite nanoparticles replaced by Sr2+, BP.JP - associated scholarships

Abstract

Recent efforts on the field of Tissue Engineering to develop resorbable bone scaffolds that mimic the structure and function of natural bone have been reported. A good bone substitute material has to reach minimum requirements such as: mechanical properties similar to target tissue, in addition to stimulate biochemical reactions fundamentals to osteointegration and osteoconduction. In this sense, composites composed of organic matrices and biominerals that are capable of mimicking bones can reach these requirements. Starch is an interesting alternative for the production of biomaterials. In addition, starch was already used for 3D printing. However, native starches have processing difficulties, high swelling, highly hydrophilic character, low mechanical resistance and instability for long-term application. In order to overcome these obstacles, methods of modifying starch have been useful for improving its functionality. As an example, starches modified by ozonation and dry heating have shown improved printability. The production via 3D printing of bone scaffolds using this modified starch is innovative and should combine its already known biocompatibility and mechanical properties with the bioactivity of biominerals. Thus, the aim of this project is to modify starches by green methods (ozonation, dry heating and moist heating) and then to evaluate their application as bone scaffolds. It will be evaluated the formulations based on modified starch, in which hydroxyapatite nanoparticles will be replaced by strontium, since the presence of this cation has been associated with the process regulation of bone formation and resorption. The production of bone scaffolds via 3D printing by extrusion can lead to materials with improved properties and also customized structure, thus, a unique. The mechanical properties of scaffolds will be evaluated simultaneously with their surface properties, combined with osteoblast and osteoclast cultivation. The cultivation of osteogenic cells on the 3D matrix, very likely, will assist to predict their tissue regenerative properties in vivo. Finally, this project will correlate aspects such as different formulations and innovative processing (3D printing) to obtain bone scaffolds with bioactive properties. This project can expand the application of starch, in particular as personalized biomaterials with unique properties, adding value to the starch chain and mainly a noble use (human health). (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANIGLIA, BIANCA CHIEREGATO; ARIAS LA FUENTE, CARLA IVONNE; SIQUEIRA, LARISSA DO VAL; TADINI, CARMEN CECILIA. Carbohydrate Nanomaterials Addition to Starch-Based Packaging: A Review about Fundamentals and Application. STARCH-STÄRKE, v. 73, n. 11-12, SI, JUL 2021. (19/21700-7, 17/05307-8, 20/08727-0, 13/07914-8, 21/05947-2)

NOGUEIRA, LUCAS FABRICIO BAHIA; EUFRASIO CRUZ, MARCOS ANTONIO; AGUILAR, GUILHERME JOSE; TAPIA-BLACIDO, DELIA RITA; DA SILVA FERREIRA, MARCIA ELIANA; MANIGLIA, BIANCA CHIEREGATO; BOTTINI, MASSIMO; CIANCAGLINI, PIETRO; RAMOS, ANA PAULA. Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 13, p. 22-pg., 2022-07-01. (18/25871-8, 20/08727-0, 16/25955-1, 19/25054-2, 21/05947-2)

SILVA, LUCAS SANTOS; DE MELO, MARYANNE TRAFANI; SPONCHIADO, PEDRO AUGUSTO INVERNIZZI; BARBOSA JUNIOR, FERNANDO; TAPIA-BLACIDO, DELIA RITA; CIANCAGLINI, PIETRO; RAMOS, ANA PAULA; MANIGLIA, BIANCA CHIEREGATO. Synthesis of composite corn starch/hydroxyapatite nanoparticle biomembranes and their effect on mineralization by osteoblasts. Journal of Applied Polymer Science, v. 140, n. 43, p. 14-pg., 2023-08-18. (19/08568-2, 21/08359-4, 20/08727-0, 19/25054-2, 21/05947-2)

MADALOSSO, HELOISA BREMM; GUINDANI, CAMILA; MANIGLIA, BIANCA CHIEREGATO; DE ARAUJO, PEDRO HENRIQUE HERMES; SAYER, CLAUDIA. Collagen-decorated electrospun scaffolds of unsaturated copolyesters for bone tissue regeneration. JOURNAL OF MATERIALS CHEMISTRY B, v. 12, n. 12, p. 16-pg., 2024-02-20. (20/08727-0)

SPONCHIADO, PEDRO AUGUSTO INVERNIZZI; DE MELO, MARYANNE TRAFANI; BITENCOURT, BRUNA SOUSA; GUEDES, JAQUELINE SOUZA; TAPIA-BLACIDO, DELIA RITA; AUGUSTO, PEDRO ESTEVES DUARTE; RAMOS, ANA PAULA; MANIGLIA, BIANCA CHIEREGATO. Clean modification of potato starch to improve 3D printing of potential bone bio-scaffolds. EMERGENT MATERIALS, v. N/A, p. 14-pg., 2024-03-15. (21/05947-2, 21/06398-2, 20/08727-0, 19/25054-2)

BITENCOURT, B. S.; GUEDES, J. S.; SALIBA, A. S. M. C.; SARTORI, A. G. O.; TORRES, L. C. R.; AMARAL, J. E. P. G.; ALENCAR, S. M.; MANIGLIA, B. C.; AUGUSTO, P. E. D.. Mineral bioaccessibility in 3D printed gels based on milk/starch/κ-carrageenan for dysphagic people. Food Research International, v. 170, p. 12-pg., 2023-05-29. (19/05043-6, 21/06398-2, 20/08727-0)

NOGUEIRA, LUCAS FABRICIO BAHIA; MANIGLIA, BIANCA C.; BUCHET, RENE; MILLAN, JOSE LUIS; CIANCAGLINI, PIETRO; BOTTINI, MASSIMO; RAMOS, ANA PAULA. hree-dimensional cell-laden collagen scaffolds: From biochemistry to bone bioengineerin. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 110, n. 4, NOV 2021. (19/08568-2, 20/08727-0, 19/25054-2, 18/25871-8)

SPONCHIADO, PEDRO AUGUSTO INVERNIZZI; MELO, MARYANNE TRAFANI; COMINAL, JUCARA G.; MARTELLI TOSI, MILENA; CIANCAGLINI, PIETRO; RAMOS, ANA PAULA; MANIGLIA, BIANCA CHIEREGATO. Biomembranes Based on Potato Starch Modified by Dry Heating Treatment: One Sustainable Strategy to Amplify the Use of Starch as a Biomaterial. Biomacromolecules, v. 26, n. 3, p. 11-pg., 2025-02-24. (23/17512-6, 19/25054-2, 20/08727-0, 19/08568-2, 21/05947-2, 22/04885-6)

SANTANA, RENATA F.; LIMA, CLARA MARIANA G.; ALVES, ANNIE N.; MANIGLIA, BIANCA C.; DOS ANJOS, LAIZA; PIRES, ANA CLARISSA S.; SANTOS, LEANDRO SOARES; RODRIGUES, LUCIANO B.; FONTAN, RAFAEL C. I.; GONCALVES, JAQUELINE TEIXEIRA T.; et al. Sorbitol-plasticized jackfruit starch-based films: investigation of the effect of the starch and plasticizer concentration on the film properties. POLYMER BULLETIN, v. 81, n. 14, p. 23-pg., 2024-06-03. (20/08727-0)

DE FARIAS, PATRICIA MARQUES; MATHEUS, JULIA RABELO VAZ; MANIGLIA, BIANCA CHIEREGATO; LE-BAIL, PATRICIA; LE-BAIL, ALAIN; SCHMID, MARKUS; FAI, ANA ELIZABETH CAVALCANTE. Bibliometric mapping analysis of Pickering emulsion applied in 3D food printing. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, v. 59, n. 4, p. 11-pg., 2024-03-03. (21/05947-2, 20/08727-0)

NOGUEIRA, LUCAS FABRICIO BAHIA; CRUZ, MARCOS ANTONIO EUFRASIO; MELO, MARYANNE TRAFANI DE; MANIGLIA, BIANCA CHIEREGATO; CAROLEO, FABRIZIO; PAOLESSE, ROBERTO; LOPES, HELENA BACHA; BELOTI, MARCIO M.; CIANCAGLINI, PIETRO; RAMOS, ANA PAULA; et al. Collagen/Kappa-Carrageenan-Based Scaffolds as Biomimetic Constructs for In Vitro Bone Mineralization Studies. Biomacromolecules, v. 24, n. 3, p. 9-pg., 2023-02-14. (19/08568-2, 20/08727-0, 22/01219-5, 19/25054-2, 16/25955-1, 18/25871-8, 21/05947-2)

LA FUENTE, CARLA IVONNE ARIAS; MANIGLIA, BIANCA CHIEREGATO; TADINI, CARMEN CECILIA. Biodegradable polymers: A review about biodegradation and its implications and applications. Packaging Technology and Science, v. 36, n. 2, p. 15-pg., 2022-11-26. (20/08727-0, 17/05307-8, 13/07914-8)

DE OLIVEIRA SARTORI, ALAN GIOVANINI; MARTELLI CHAIB SALIBA, ANA SOFIA; BITENCOURT, BRUNA SOUSA; GUEDES, JAQUELINE SOUZA; ROCHA TORRES, LARISSA CATELLI; DE ALENCAR, SEVERINO MATIAS; AUGUSTO, PEDRO ESTEVES DUARTE. Anthocyanin bioaccessibility and anti-inflammatory activity of a grape-based 3D printed food for dysphagia. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 84, p. 13-pg., 2023-01-28. (19/05043-6, 20/08727-0, 21/06398-2)