Maresin-1 and Resolvin E1 Promote Regenerative Properties of Periodontal Ligament Stem Cells Under Inflammatory Conditions

Author(s)

Albuquerque-Souza, Emmanuel ; Schulte, Fabian ; Chen, Tsute ; Hardt, Markus ; Hasturk, Hatice ; Van Dyke, Thomas E. ; Holzhausen, Marinella ; Kantarci, Alpdogan

• Document type: Journal article
• Source: FRONTIERS IN IMMUNOLOGY; v. 11, p. 15-pg., 2020-09-25.

Abstract

Maresin-1 (MaR1) and Resolvin E1 (RvE1) are specialized pro-resolving lipid mediators (SPMs) that regulate inflammatory processes. We have previously demonstrated the hard and soft tissue regenerative capacity of RvE1 in anin vivomodel of the periodontal disease characterized by inflammatory tissue destruction. Regeneration of periodontal tissues requires a well-orchestrated process mediated by periodontal ligament stem cells. However, limited data are available on how SPMs can regulate the regenerative properties of human periodontal ligament stem cells (hPDLSCs) under inflammatory conditions. Thus, we measured the impact of MaR1 and RvE1 in anin vitromodel of hPDLSC under stimulation with IL-1 beta and TNF-alpha by evaluating pluripotency, migration, viability/cell death, periodontal ligament markers (alpha-smooth muscle actin, tenomodulin, and periostin), cementogenic-osteogenic differentiation, and phosphoproteomic perturbations. The data showed that the pro-inflammatory milieu suppresses pluripotency, viability, and migration of hPDLSCs; MaR1 and RvE1 both restored regenerative capacity by increasing hPDLSC viability, accelerating wound healing/migration, and up-regulating periodontal ligament markers and cementogenic-osteogenic differentiation. Protein phosphorylation perturbations were associated with the SPM-induced regenerative capacity of hPDLSCs. Together, these results demonstrate that MaR1 and RvE1 restore or improve the regenerative properties of highly specialized stem cells when inflammation is present and offer opportunities for direct pharmacologic treatment of lost tissue integrity. (AU)

• FAPESP's process: 17/25260-6 - THE ACTION OF MARESIN 1 (MAR1) ON FIBROGENESIS AND OSTEOGENIC ACTIVITY OF HUMAN PERIODONTAL LIGAMENT CELLS
• Grantee: Emmanuel Albuquerque de Souza
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate