Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus

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Bertolini, Amanda Bezerra ; Thyssen, Patricia Jacqueline ; Brandao, Paulo Eduardo ; Prado, Aline Marrara ; Silva, Sheila Oliveira de Souza ; Mioni, Mateus de Souza Ribeiro ; de Gouvea, Fabio Lucas Rezende ; Pantoja, Jose Carlos de Figueiredo ; Langoni, Helio ; Guimaraes, Felipe Freitas ; Joaquim, Samea Fernandes ; Guerra, Simony Trevizan ; Leite, Domingos da Silva ; Rall, Vera Mores ; Hernandes, Rodrigo Tavanelli ; Lucheis, Simone Baldini ; Rossi, Gabriel Augusto Marques ; Ribeiro, Marcio Garcia

• Document type: Journal article
• Source: Journal of Applied Microbiology; v. N/A, p. 6-pg., 2023-02-01.

Abstract

Aims We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms. Methods and Results We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens. Conclusions Our findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health. (AU)

• FAPESP's process: 15/19688-8 - E. coli, Klebsiella pnemoniae and Enterococcus spp: impact of virulence factors in bovine mastitis and public health concern
• Grantee: Helio Langoni
• Support Opportunities: Research Projects - Thematic Grants