Lactobacilli postbiotics reduce biofilm formation and alter transcription of virulence genes of Aggregatibacter actinomycetemcomitans

Author(s)

Ishikawa, Karin H. ; Bueno, Manuela R. ; Kawamoto, Dione ; Simionato, Maria R. L. ; Mayer, Marcia P. A.

• Document type: Journal article
• Source: Molecular Oral Microbiology; v. 36, n. 1, p. 11-pg., 2021-01-17.

Abstract

Periodontitis is characterized by a dysbiotic microbial community and treatment strategies include the reestablishment of symbiosis by reducing pathogens abundance. Aggregatibacter actinomycetemcomitans (Aa) is frequently associated with rapidly progressing periodontitis. Since the oral ecosystem may be affected by metabolic end-products of bacteria, we evaluated the effect of soluble compounds released by probiotic lactobacilli, known as postbiotics, on Aa biofilm and expression of virulence-associated genes. Cell-free pH-neutralized supernatants (CFS) of Lactobacillus rhamnosus Lr32, L. rhamnosus HN001, Lactobacillus acidophilus LA5, and L. acidophilus NCFM were tested against a fimbriated clinical isolate of Aa JP2 genotype (1 x 10(7) CFU/well) on biofilm formation for 24 hr, and early and mature preformed biofilms (2 and 24 hr). Lactobacilli CFS partially reduced Aa viable counts and biofilms biomass, but did not affect the number of viable non-adherent bacteria, except for LA5 CFS. Furthermore, LA5 CFS and, in a lesser extent HN001 CFS, influenced Aa preformed biofilms. Lactobacilli postbiotics altered expression profile of Aa in a strain-specific fashion. Transcription of cytolethal distending toxin (cdtB) and leukotoxin (ltxA) was downregulated by CFS of LA5 and LR32 CFS. Although all probiotics produced detectable peroxide, transcription of katA was downregulated by lactobacilli CFS. Transcription of dspB was abrogated by LR32 and NCFM CFS, but increased by HN001, whereas expression of pgA was not affected by any postbiotic. Our data indicated the potential of postbiotics from lactobacilli, especially LA5, to reduce colonization levels of Aa and to modulate the expression of virulence factors implicated in evasion of host defenses. (AU)

• FAPESP's process: 15/18273-9 - New strategies for the control of periodontitis
• Grantee: Marcia Pinto Alves Mayer
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/16377-7 - EVALUATION OF THE EFFECT OF PROBIOTIC BACTERIA ON THE IMUNE RESPONSE TO A.ACTINOMYCETEMCOMITANS: A STUDY IN GINGIVAL EPITHELIAL CELLS AND IN EXPERIMENTAL MODEL OF INFECTION IN VIVO.
• Grantee: Manuela Rocha Bueno
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/13156-7 - Direct and immunomodulatory effects of probiotic bacteria on periodontal pathogens
• Grantee: Karin Hitomi Ishikawa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral