| Full text | |
| Author(s): |
Moya, Eva Balaguer
;
Silva Cunha, Maria Laura
;
Prado, Carina Aline
;
Turella, Simone
;
da Silva, Silvio Silverio
;
Abou-Hachem, Maher
;
Dragone, Giuliano
;
dos Santos, Julio Cesar
;
Mussatto, Solange Ines
Total Authors: 9
|
| Document type: | Journal article |
| Source: | CATALYSTS; v. 12, n. 10, p. 10-pg., 2022-10-01. |
| Abstract | |
This study evaluated innovative approaches for the enzymatic hydrolysis of lignocellulosic biomass. More specifically, assays were performed to evaluate the supplementation of the commercial cellulolytic cocktail Cellic(R) CTec2 (CC2) with LPMO (GcLPMO9B), H2O2, or cello-oligosaccharide dehydrogenase (CelDH) FgCelDH7C in order to boost the LPMO action and improve the saccharification efficiency of biomass into monosaccharides. The enzymatic hydrolysis was carried out using sugarcane bagasse pretreated by hydrodynamic cavitation-assisted oxidative process, 10% (w/w) solid loading, and 30 FPU CC2/g dry biomass. The results were compared in terms of sugars release and revealed an important influence of the supplementations at the initial 6 h of hydrolysis. While the addition of CelDH led to a steady increase in glucose production to reach 101.1 mg of glucose/g DM, accounting for the highest value achieved after 72 h of hydrolysis, boosting the LPMOs activity by the supplementation of pure LPMOs or the LPMO co-substrate H2O2 were also effective to improve the cellulose conversion, increasing the initial reaction rate of the hydrolysis. These results revealed that LPMOs play an important role on enzymatic hydrolysis of cellulose and boosting their action can help to improve the reaction rate and increase the hydrolysis yield. LPMOs-CelDH oxidative pairs represent a novel potent combination for use in the enzymatic hydrolysis of lignocellulose biomass. Finally, the strategies presented in this study are promising approaches for application in lignocellulosic biorefineries, especially using sugarcane bagasse as a feedstock. (AU) | |
| FAPESP's process: | 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales |
| Grantee: | Roberto de Campos Giordano |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 20/12059-3 - Alternative technologies for ethanol and pullulan production in sugarcane bagasse biorefineries using hydrodynamic cavitation reactors and systems for simultaneous hydrolysis and fermentation |
| Grantee: | Júlio César dos Santos |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 20/16638-8 - Evaluation of 2G ethanol production from sugarcane bagasse pretreated by hydrodynamic cavitation-assisted oxidative process: pretreatment conditions and utilization of simultaneous hydrolysis and fermentation reactors with interconnected columns system |
| Grantee: | Maria Laura Silva Cunha |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |