| Full text | |
| Author(s): |
Valduga, Amanda Harduim
;
Mizobuti, Daniela Sayuri
;
Moraes, Fernanda dos Santos Rapucci
;
Mancio, Rafael Dias
;
Moraes, Luis Henrique Rapucci
;
Hermes, Tulio de Almeida
;
Macedo, Aline Barbosa
;
Minatel, Elaine
Total Authors: 8
|
| Document type: | Journal article |
| Source: | International Journal of Experimental Pathology; v. 104, n. 1, p. 9-pg., 2022-12-24. |
| Abstract | |
There is strong cross-talk between abnormal intracellular calcium concentration, high levels of reactive oxygen species (ROS) and an exacerbated inflammatory process in the dystrophic muscles of mdx mice, the experimental model of Duchenne muscular dystrophy (DMD). In this study, we investigated effects of Idebenone, a potent anti-oxidant, on oxidative stress markers, the anti-oxidant defence system, intracellular calcium concentrations and the inflammatory process in primary dystrophic muscle cells from mdx mice. Dystrophic muscle cells were treated with Idebenone (0.05 mu M) for 24 h. The untreated mdx muscle cells were used as controls. The MTT assay showed that Idebenone did not have a cytotoxic effect on the dystrophic muscle cells. The Idebenone treatment was able to reduce the levels of oxidative stress markers, such as H2O2 and 4-HNE, as well as decreasing intracellular calcium influx in the dystrophic muscle cells. Regarding Idebenone effects on the anti-oxidant defence system, an up-regulation of catalase levels, glutathione reductase (GR), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity was observed in the dystrophic muscle cells. In addition, the Idebenone treatment was also associated with reduction in inflammatory molecules, such as nuclear factor kappa-B (NF-kappa B) and tumour necrosis factor (TNF) in mdx muscle cells. These outcomes supported the use of Idebenone as a protective agent against oxidative stress and related signalling mechanisms involved in dystrophinopathies, such as DMD. (AU) | |
| FAPESP's process: | 17/01638-0 - TEMPOL: POTENTIAL ANTIOXIDANT THERAPY FOR DYSTROPHIC MUSCULAR FIBERS |
| Grantee: | Elaine Minatel |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 20/09733-4 - Effect of photobiomodulation and antioxidant therapies on cellular, tissue, molecular and biochemical events on dystrophic muscle fibers in Mdx mice |
| Grantee: | Elaine Minatel |
| Support Opportunities: | Regular Research Grants |