Effect of photobiomodulation and antioxidant therapies on cellular, tissue, molecular and biochemical events on dystrophic muscle fibers in Mdx mice

• Grant number: 20/09733-4
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2021 - May 31, 2023
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Elaine Minatel
• Grantee: Elaine Minatel
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Elaine Cristina Leite Pereira ; Leonardo dos Reis Silveira ; Valéria Helena Alves Cagnon Quitete

Abstract

The administration of glucorticoids is currently the standard pharmacological treatment prescribed for patients with Duchenne muscular disease (DMD). Due to its side effects, the search for alternative therapies that minimize the evolution of the disease and improve the quality of life of dystrophic patients is necessary. In this context, we hypothesized that the combination of photobiomodulation and antioxidant therapies may have a potential beneficial effect on cellular, tissue, molecular and biochemical events of dystrophic muscle fiber. Thus, the present study aims to analyze "in vitro" and "in vivo" the effects of the combined therapy of LED and Idebenone on the dystrophic muscle fibers of mdx mice, an experimental model of DMD. In the "in vitro" study, the combination of LED therapy (LEDT) at a wavelength of 850 nm and the antioxidant Idebenone (0.5µM) on primary muscle cell cultures of mdx mice will be analyzed. After 48 hours of treatment, the dystrophic muscle cells will be subjected to biochemical and molecular analyzes. For the "in vivo" study mdx mice will be used, with 14 days of postnatal life, submitted to the application of LEDT light in the middle third of the quadriceps muscle (3x / week) and treated with Idebenone (200mg / kg, daily) for a two-week period. After treatment, the quadriceps muscle will be removed and used for morphological, biochemical and molecular analyzes. (AU)