Effect of photobiomodulation and antioxidant therapies on cellular, tissue, molecular and biochemical events on dystrophic muscle fibers in Mdx mice

• Grant number: 20/09733-4
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2021
• End date: May 31, 2023
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Elaine Minatel
• Grantee: Elaine Minatel
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Elaine Cristina Leite Pereira ; Leonardo dos Reis Silveira ; Valéria Helena Alves Cagnon Quitete

Abstract

The administration of glucorticoids is currently the standard pharmacological treatment prescribed for patients with Duchenne muscular disease (DMD). Due to its side effects, the search for alternative therapies that minimize the evolution of the disease and improve the quality of life of dystrophic patients is necessary. In this context, we hypothesized that the combination of photobiomodulation and antioxidant therapies may have a potential beneficial effect on cellular, tissue, molecular and biochemical events of dystrophic muscle fiber. Thus, the present study aims to analyze "in vitro" and "in vivo" the effects of the combined therapy of LED and Idebenone on the dystrophic muscle fibers of mdx mice, an experimental model of DMD. In the "in vitro" study, the combination of LED therapy (LEDT) at a wavelength of 850 nm and the antioxidant Idebenone (0.5µM) on primary muscle cell cultures of mdx mice will be analyzed. After 48 hours of treatment, the dystrophic muscle cells will be subjected to biochemical and molecular analyzes. For the "in vivo" study mdx mice will be used, with 14 days of postnatal life, submitted to the application of LEDT light in the middle third of the quadriceps muscle (3x / week) and treated with Idebenone (200mg / kg, daily) for a two-week period. After treatment, the quadriceps muscle will be removed and used for morphological, biochemical and molecular analyzes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA ROCHA, GUILHERME LUIZ; MIZOBUTI, DANIELA SAYURI; MARIANO DA SILVA, HELOINA NATHALLIE; COVATTI, CAROLINE; DE LOURENCO, CAROLINE CARAMANO; SALVADOR, MARCOS JOSE; LEITE PEREIRA, ELAINE CRISTINA; MINATEL, ELAINE. Multiple LEDT wavelengths modulate the Akt signaling pathways and attenuate pathological events in mdx dystrophic muscle cells. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. N/A, p. 16-pg., 2022-04-05. (20/09733-4)

BORGHI, ALEXANDRE AUGUSTO; MINATEL, ELAINE; MIZOBUTI, DANIELA SAYURI; DE LOURENCO, CAROLINE CARAMANO; DE ARAUJO, FABIO FERNANDES; PASTORE, GLAUCIA MARIA; HEWITSON, PETER; IGNATOVA, SVETLANA; SAWAYA, ALEXANDRA C. H. F.. Antioxidant and Anti-inflammatory Activity of Mikania glomerata and Mikania laevigata Extracts. PHARMACOGNOSY RESEARCH, v. 15, n. 1, p. 10-pg., 2023-01-01. (15/50333-1, 20/09733-4, 20/15163-6)

VALDUGA, AMANDA HARDUIM; MIZOBUTI, DANIELA SAYURI; MORAES, FERNANDA DOS SANTOS RAPUCCI; MANCIO, RAFAEL DIAS; MORAES, LUIS HENRIQUE RAPUCCI; HERMES, TULIO DE ALMEIDA; MACEDO, ALINE BARBOSA; MINATEL, ELAINE. Protection of dystrophic muscle cells using Idebenone correlates with the interplay between calcium, oxidative stress and inflammation. International Journal of Experimental Pathology, v. 104, n. 1, p. 9-pg., 2022-12-24. (17/01638-0, 20/09733-4)