Biosynthesis of silver nanoparticles from Syzygium cumini leaves and their potential effects on odontogenic pathogens and biofilms

Author(s)

Bernardo, Wagner Luis de Carvalho ; Boriollo, Marcelo Fabiano Gomes ; Tonon, Caroline Coradi ; da Silva, Jeferson Junior ; Oliveira, Mateus Cardoso ; de Moraes, Fernando Cruz ; Spolidorio, Denise Madalena Palomari

• Document type: Journal article
• Source: FRONTIERS IN MICROBIOLOGY; v. 13, p. 22-pg., 2022-09-29.

Abstract

This study analyzed the antimicrobial and antibiofilm action and cytotoxicity of extract (HEScL) and silver nanoparticles (AgNPs-HEScL) from Syzygium cumini leaves. GC-MS, UV-Vis, EDX, FEG/SEM, DLS and zeta potential assays were used to characterize the extract or nanoparticles. Antimicrobial, antibiofilm and cytotoxicity analyses were carried out by in vitro methods: agar diffusion, microdilution and normal oral keratinocytes spontaneously immortalized (NOK-SI) cell culture. MICs of planktonic cells ranged from 31.2-250 (AgNPs-HEScL) to 1,296.8-10,375 mu g/ml (HEScL) for Actinomyces naeslundii, Fusobacterium nucleatum, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Streptococcus oralis, Veillonella dispar, and Candida albicans. AgNPs-HEScL showed antibiofilm effects (125-8,000 mu g/ml) toward Candida albicans, Streptococcus mutans and Streptococcus oralis, and Staphylococcus aureus and Staphylococcus epidermidis. The NOK-SI exhibited no cytotoxicity when treated with 32.8 and 680.3 mu g/ml of AgNPs-HEScL and HEScL, respectively, for 5 min. The data suggest potential antimicrobial and antibiofilm action of HEScL, and more specifically, AgNPs-HEScL, involving pathogens of medical and dental interest (dose-, time- and species-dependent). The cytotoxicity of HEScL and AgNPs-HEScL detected in NOK-SI was dose- and time-dependent. This study presents toxicological information about the lyophilized ethanolic extract of S. cumini leaves, including their metallic nanoparticles, and adds scientific values to incipient studies found in the literature. (AU)

• FAPESP's process: 22/05454-9 - Development of disposable electrochemical sensors and photoanodes manufactured with nanostructured semiconductor materials for the determination and degradation of endocrine disruptors
• Grantee: Fernando Cruz de Moraes
• Support Opportunities: Regular Research Grants