Experimental Apparatus for Evaluation of Calcium Fluctuations in Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells

Author(s):Show less -

Arana, M. C. ; Lahuerta, R. D. ; Cortella, L. R. X. ; Mazzetto, M. ; Soldera, M. ; Lasagni, A. F. ; Cestari, I. N. ; Cestari, I. A. ; Bastos-Filho, TF ; Caldeira, EMD ; Frizera-Neto, A

• Document type: Journal article
• Source: XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020; v. N/A, p. 5-pg., 2022-01-01.

Abstract

In this work, we developed and tested an experimental apparatus to evaluate calcium fluctuations in cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CM). The set-up is composed of a signal module for registration and analysis of the signals and a perfusion chamber. This chamber allows the maintenance of the cells, control of perfusion and temperature, and electric stimulation. The signal module consists of a CCD camera attached to a fluorescence microscope with the appropriate software and hardware for eliciting and recording fluorescent signals originating from hiPSC-CM intracellular Ca+2 concentration changes, under electrical stimulation. We employed this system for analysis of calcium fluctuation from hiPSC-CM cultivated on micro textured polyethylene terephthalate surfaces. (AU)

• FAPESP's process: 12/50283-6 - Circulatory support in pediatric patients: ventricular assist device and extracorporeal membrane oxygenation
• Grantee: Idágene Aparecida Cestari
• Support Opportunities: Research Projects - Thematic Grants