First description of Giardia duodenalis in buffalo calves (Bubalus bubalis) in southwest region of Sao Paulo State, Brazil

Author(s)

Costa de Aquino, Monally Conceicao ; Harvey, Tatiani Vitor ; Inacio, Sandra Valeria ; Nagata, Walter Bertequini ; Ferrari, Elis Domingos ; Miranda Oliveira, Bruno Cesar ; Albuquerque, George Rego ; Widmer, Giovanni ; Meireles, Marcelo Vasconcelos ; Saraiva Bresciani, Katia Denise

• Document type: Journal article
• Source: FOOD AND WATERBORNE PARASITOLOGY; v. 16, p. 5-pg., 2019-09-01.

Abstract

We performed molecular characterization of Giardia duodenalis in buffalo calves from the Southwest region of Sao Paulo State, Brazil. A total of 183 fecal samples of Murrah breed buffaloes up to six months of age were collected. We examined these samples by the polymerase chain reaction (PCR) targeting the small-subunit ribosomal RNA gene and positive samples were characterized using additional PCR assays targeting a portion of the beta-giardin, the glutamate dehydrogenase and the triose-phosphate isomerase genes. Based on the SSU rRNA nPCR, the presence of G. duodenalis was confirmed in 12 (6.56%) of fecal samples, of these, five, four and three samples were positive for the tpi, bg and gdh genes, respectively. Assemblage identification by sequencing was successful in 6 of 12 samples and sequence analysis showed 100% genetic similarity with G. duodenalis assemblage E. This observation represents the first detection of G. duodenalis assemblage E in buffaloes calves in Brazil. (C) 2019 Published by Elsevier Inc. on behalf of International Association of Food and Waterborne Parasitology. (AU)

• FAPESP's process: 10/52542-3 - Occurrence of the infection by Cryptosporidium spp. in water buffaloes, goats and sheeps
• Grantee: Katia Denise Saraiva Bresciani
• Support Opportunities: Regular Research Grants