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Effect of season on oocyte quality of buffaloes (Bubalus bubalis): analysis of gene expression and mitochondrial DNA copy number

Grant number: 11/14207-0
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2011
Effective date (End): December 31, 2012
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Marcos Roberto Chiaratti
Grantee:Carolina Habermann Macabelli
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil


In Brazil, as well as in many other countries, the buffalo herd has been showing rapid growth due economic interests regarding meat and milk production. As seen in other domestic animals, this expansion is supported by the genetic improvement, which in turn depends on several biotechnologies, including the in vitro embryo production. The latter is seen an important tool for enhancing the reproductive efficiency of female buffaloes, however, oocytes used for in vitro embryo production have their quality strongly influenced by environmental factors such as photoperiod, environmental temperature, nutrition among others. Since female buffaloes are seasonally polyestrous, there is a marked reduction in their fertility during the summer in regions of high latitude. However, this environmental effect on buffalo fertility does not appear to be due to a failure on follicular development, but due to an impairment of oocyte developmental competence. The factors determining this impairment are unknown though. Therefore, this project aims to investigate the effect of reproductive seasonality and the number of calving on gene expression (genes related to heat stress, oxidative stress and metabolism) and on the number of mitochondrial DNA copies in oocytes of two buffalo categories, nulliparous and multiparous, collected during the winter and the summer. Oocyte collection will be carried out in Registro/SP, using animals from the Agência Paulista de Tecnologia dos Agronegócios (APTA), because the reproductive seasonality of these female buffaloes has been well characterized. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA, ROBERTA MACHADO; CHIARATTI, MARCOS ROBERTO; MACABELLI, CAROLINA HABERMANN; RODRIGUES, CARLOS ALBERTO; FERRAZ, MARCIO LEAO; WATANABE, YEDA FUMIE; SMITH, LAWRENCE CHARLES; MEIRELLES, FLAVIO VIEIRA; BARUSELLI, PIETRO SAMPAIO. The Infertility of Repeat-Breeder Cows During Summer Is Associated with Decreased Mitochondrial DNA and Increased Expression of Mitochondrial and Apoptotic Genes in Oocytes. BIOLOGY OF REPRODUCTION, v. 94, n. 3, . (09/00938-3, 10/13384-3, 11/14207-0, 10/09561-7, 12/07510-1)
MACABELLI, CAROLINA HABERMANN; FERREIRA, ROBERTA MACHADO; GIMENES, LINDSAY UNNO; TONIZZA DE CARVALHO, NELCIO ANTONIO; SOARES, JULIA GLEYCI; AYRES, HENDERSON; FERRAZ, MARCIO LEAO; WATANABE, YEDA FUMIE; WATANABE, OSNIR YOSHIME; SANGALLI, JULIANO RODRIGUES; et al. Reference Gene Selection for Gene Expression Analysis of Oocytes Collected from Dairy Cattle and Buffaloes during Winter and Summer. PLoS One, v. 9, n. 3, . (09/00938-3, 10/13384-3, 11/14207-0, 10/09561-7, 12/07510-1)

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