Effect of season on oocyte quality and in vitro embryo production in Holstein cows (Bos taurus) of different categories

• Grant number: 09/00938-3
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2009
• End date: November 30, 2011
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Pietro Sampaio Baruselli
• Grantee: Pietro Sampaio Baruselli
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The use of in vitro embryo production (IVP) technique to improve the reproductive capacity of the domestic animals has been substantially important for animal production. However, the IVP is still very inefficient for high producing Holstein cows and influenced by many factors. The environmental conditions to which the animals are submitted to is one important factor to be considered. Moreover, the category of the animal used as oocyte donor can also be decisive for IVP success. Thus, in the present proposal, the effect of the season of the year (warm and humid x fresh) will be studied in different animal categories (high-producing lactating cows, repeat breeder lactating cows and heifers) in a commercial Holstein (Bos taurus)herd. The main objective of the present experiment is to determine the best season and categories of Holstein females to be submitted to follicular aspiration for in vitro embryo production, based on physiological and molecular analysis. First, the physiological parameters, the recovery rate and the rate of viable oocytes recovered by in vivo ovum pick-up (OPU) technique will be analyzed. After, the quality of these recovered oocytes will be evaluated by the expression of genes related to heat stress (HSP70 e HSP90) and apoptosis (Bax, Bcl2 e ITM2B) and by quantitative mitochondrial DNA (mtDNA). Additionally, the rates related to in vitro embryo development after in vitro fertilization and the blastocysts quality ( total number of cells and frequency of apoptotic nuclei) will be evaluated. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, ROBERTA MACHADO; CHIARATTI, MARCOS ROBERTO; MACABELLI, CAROLINA HABERMANN; RODRIGUES, CARLOS ALBERTO; FERRAZ, MARCIO LEAO; WATANABE, YEDA FUMIE; SMITH, LAWRENCE CHARLES; MEIRELLES, FLAVIO VIEIRA; BARUSELLI, PIETRO SAMPAIO. The Infertility of Repeat-Breeder Cows During Summer Is Associated with Decreased Mitochondrial DNA and Increased Expression of Mitochondrial and Apoptotic Genes in Oocytes. BIOLOGY OF REPRODUCTION, v. 94, n. 3, MAR 1 2016. (09/00938-3, 10/13384-3, 11/14207-0, 10/09561-7, 12/07510-1)

MACABELLI, CAROLINA HABERMANN; FERREIRA, ROBERTA MACHADO; GIMENES, LINDSAY UNNO; TONIZZA DE CARVALHO, NELCIO ANTONIO; SOARES, JULIA GLEYCI; AYRES, HENDERSON; FERRAZ, MARCIO LEAO; WATANABE, YEDA FUMIE; WATANABE, OSNIR YOSHIME; SANGALLI, JULIANO RODRIGUES; et al. Reference Gene Selection for Gene Expression Analysis of Oocytes Collected from Dairy Cattle and Buffaloes during Winter and Summer. PLoS One, v. 9, n. 3, MAR 27 2014. (09/00938-3, 10/13384-3, 11/14207-0, 10/09561-7, 12/07510-1)

FERREIRA, R. M.; AYRES, H.; CHIARATTI, M. R.; FERRAZ, M. L.; ARAUJO, A. B.; RODRIGUES, C. A.; WATANABE, Y. F.; VIREQUE, A. A.; JOAQUIM, D. C.; SMITH, L. C.; et al. The low fertility of repeat-breeder cows during summer heat stress is related to a low oocyte competence to develop into blastocysts. JOURNAL OF DAIRY SCIENCE, v. 94, n. 5, p. 2383-2392, MAY 2011. (09/00938-3)

FERREIRA, R. M.; AYRES, H.; GIMENES, L. U.; TORRES, F. P.; LIMA, F. A.; VERAS, M. B.; GUIDA, T. G.; SALA, R. V.; BARUSELLI, P. S.. Inducing ovulation with oestradiol cypionate allows flexibility in the timing of insemination and removes the need for gonadotrophin-releasing hormone in timed AI protocols for dairy cows. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 29, n. 3, p. 468-475, 2017. (09/00938-3, 12/07510-1)

BARUSELLI, PIETRO S.; FERREIRA, ROBERTA M.; VIEIRA, LAIS M.; SOUZA, ALEXANDRE H.; BO, GABRIEL A.; RODRIGUES, CARLOS A.. Use of embryo transfer to alleviate infertility caused by heat stress. Theriogenology, v. 155, p. 1-11, OCT 1 2020. (09/00938-3, 12/07510-1)