Delivery of superoxide dismutase by TAT and abalone peptides for the protection of skin cells against oxidative stress

Author(s)

Reigado, Gustavo Roncoli ; Adriani, Patricia Pereira ; dos Santos, Jeniffer Farias ; Freitas, Bruna Leticia ; Pruschinski Fernandes, Myrian Thiago ; Chambergo Alcalde, Felipe Santiago ; Leo, Patricia ; Nunes, Viviane Abreu

• Document type: Journal article
• Source: Biotechnology and Applied Biochemistry; v. 69, n. 6, p. 13-pg., 2022-02-08.

Abstract

Trichoderma reesei superoxide dismutase (TrSOD) is a well-characterized enzyme being stable between 30 and 90. C for 1 h with activity at pH between 2.6 and 9.0. This work aimed to clone, express, purify, and evaluate the protective effect antioxidant of this enzyme on skin cells when fused to transactivator of transcription (TAT) protein transduction domain of HIV-1 and abalone (Ab) peptides to allow cell penetration. TrSOD, TAT-TrSOD-Yfp (fused to yellow fluorescent protein), and Ab-TrSOD were expressed in E. coli and purified as soluble proteins. The cytotoxicity of the enzymes, at the concentrations of 1, 3, and 6 mu mol/L, was evaluated for a period of 24 and 48 h of incubation, with no cytotoxic effect on 3T3 fibroblasts. The 3T3 cells were exposed to the oxidant agent tert-butyl hydroperoxide and evaluated for reactive oxygen species (ROS) generation, in the presence or not of the recombinant enzymes. TAT-TrSOD-Yfp was able to decrease the generation of ROS by 15% when used in the concentrations of 3 and 6 mu mol/L in comparison to the control, but there was no difference in relation to the effect of TrSOD. Ab-TrSOD, when compared to TrSOD, promoted a decrease in the formation of ROS of 19% and 14% at the concentrations of 1 and 6 mu mol/L, respectively, indicating that this recombinant form was more effective in reducing oxidative stress compared to SOD without the cell-penetrating peptide (CPP). Together, these results indicate that the fusion of SOD with these CPP increased the antioxidant capacity of fibroblasts, identified by the reduction in the generation of ROS. In addition, such molecules, in the concentrations initially used, were not toxic to the cells, opening perspectives for the development of products for antioxidant protection of the skin that may have therapeutic and cosmetic application. (AU)

• FAPESP's process: 20/00030-0 - Enzyme system development with permeation technology for antioxidant protection in skin cells
• Grantee: Patricia Pereira Adriani
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• FAPESP's process: 21/09105-6 - Development of an enzymatic system with permeation technology for skin cell antioxidant protection
• Grantee: Patricia Pereira Adriani
• Support Opportunities: Scholarships in Brazil - Innovative Research in Small Business - PIPE