Integration of first- and second-generation ethanol production: Evaluation of a mathematical model to describe sucrose and xylose co-fermentation by recombinant Saccharomyces cerevisiae

Author(s)

Martinez-Jimenez, F. D. ; Pereira, I. O. ; Ribeiro, M. P. A. ; Sargo, C. R. ; dos Santos, A. A. ; Zanella, E. ; Stambuk, B. U. ; Ienczak, J. L. ; Morais, E. R. ; Costa, A. C.

• Document type: Journal article
• Source: RENEWABLE ENERGY; v. 192, p. 14-pg., 2022-05-06.

Abstract

The use of hydrolysates of lignocellulosic materials and microbial platforms present themselves as viable alternatives to the current system energy. The metabolization of the different carbohydrates present in the hydrolysates by yeasts is a process bottleneck. Genetically modified yeasts can ferment different carbohydrates. Furthermore, mathematical modelling helps to understand the dynamics and interactions during the process and predicts other conditions. The performance of the recombinant yeast Saccharomyces cerevisiae was evaluated for co-fermentation in different carbohydrates proportions (C12 - C5, 30.67 +/- 0.71-70.17 +/- 2.64%) and initial cell densities (0.28 +/- 0.07, 10.95 +/- 0.250 and 30.25 +/- 0.25 g center dot L-1) using an unstructured - unsegregated kinetic model aiming the integration of first-and-second generation ethanol production. The method to obtain confidence region, the parameter's correlation, and the convergence of the parameters followed a pipeline previously developed by our researcher group. The mathematical models described the fermentations, and mu Glu,max, mu Fru,max, lrGlu,max, lrXyl,max, rXyl,max and jXyl,max were the parameters with the slightest variation during the convergence process. Also, it was identified that the yeast consumed glucose and fructose simultaneously to form cell, however mu Glu,max was higher relative to mu Fru,max in all cases (0.1909, 0.0341, 0.1815 h-1 for mu Glu,max, and 1.0 0E-5, 2.86E-05, 2.4 4E-05 h-1 for mu Fru,max). On the other hand, lrGlu,max and lrFru,max were higher relative to lrXyl,max. (c) 2022 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/04997-0 - Scheffersomyces stipitis and Spathaspora passalidarum wild type yeast assessment for second generation bioethanol production from bagasse sugarcarne hydrolyzates containing xylose and glucose
• Grantee: Jaciane Lutz Ienczak
• Support Opportunities: Regular Research Grants