Polymorphisms at the IL17A and IL17RA Genes are Associated with Prognosis of Papillary Thyroid Carcinoma

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Bertol, Bruna Cristina ; de Araujo, Jessica Nayara Goes ; de Carvalho, Kleyton Thiago Costa ; dos Santos, Marcos Gonzaga ; Martelli-Palomino, Gustavo ; Feitosa, Nathalie Lobo de Figueiredo ; Maciel, Lea Maria Zanini ; Silbiger, Vivian Nogueira ; Lucena-Silva, Norma ; Freitas, Janaina Cristiana de Oliveira Crispim ; Donadi, Eduardo Antonio

• Document type: Journal article
• Source: ARCHIVES OF MEDICAL RESEARCH; v. 53, n. 2, p. 7-pg., 2022-02-01.

Abstract

Background. Interleukin (IL)-17A has a dual role in tumor immunity, promotes anti-tumor responses and facilitates angiogenesis by interacting with IL-17 receptor A (IL-17RA). Although IL-17A has been associated with the pathogenesis of papillary thyroid carcinoma (PTC), the nucleotide variability at the IL17A and IL17RA genes is still poorly characterized. Aim. To assess the contribution of the IL17A (-197 G > A, rs2275913) and IL17RA (-947 A > G, rs4819554) single nucleotide polymorphisms (SNP) on the development and progression of PTC and on IL-17 plasma levels. Methods. We studied 188 PTC patients and 170 healthy controls. SNPs were identi-fied using PCR-amplified DNA and restriction fragment length polymorphism (RFLP) techniques. Plasma levels of IL-17A was evaluated in 83 PTC patients using ELISA. Statistical analyses were performed to evaluate the associations between SNPs and clin-icohistopathological features of PTC and IL-17A levels. Results. No significant difference was observed regarding the allele and genotype distri-butions of both SNPs between PTC patients and controls. The IL17A GA was associated with poor biochemical and structural incomplete response to therapy, whereas no in-fluence over the IL-17A expression was observed. The IL17RA AG was significantly associated with small-sized tumors, initial tumor stage at diagnosis and better response to therapy. Conclusions. The IL17A SNP may predict an aggressive manifestation of PTC, whereas the IL17RA SNP was associated with a more favorable clinical outcome. (c) 2021 Instituto Mexicano del Seguro Social (IMSS). Published by Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 15/26556-0 - Association of HLA-G, BRAF and TERT genes with malignancy of papillary thyroid carcinoma
• Grantee: Bruna Cristina Bertol
• Support Opportunities: Scholarships in Brazil - Doctorate