Pracaxi oil affects xenobiotic metabolisms, cellular proliferation, and oxidative stress without cytotogenotoxic effects in HepG2/C3A cells

Author(s)

Pires, Camila Lehnhardt ; Zanetti, Thalita Alves ; Mantovani, Mario Sergio ; Gaivao, Isabel O'Neill de Mascarenhas ; Perazzo, Fabio Ferreira ; Rosa, Paulo Cesar Pires ; Maistro, Edson Luis

• Document type: Journal article
• Source: TOXICOLOGY IN VITRO; v. 83, p. 9-pg., 2022-06-03.

Abstract

Pentaclethra macroloba (Willd.) Kuntze seeds oil has been used as a topical healing agent, applied mainly to parturients and snake bites. The objective was to investigate the effects of pracaxi oil (POP) on HepG2/C3A cells under cytogenotoxicity, cell cycle and apoptosis influence, and expression of metabolism and other related cell types proliferation genes. Cytotoxicity was analyzed by MTT test and apoptosis and cell cycle interferences by flow cytometry. To identify genotoxicity were used comet and micronucleus tests. RT-qPCR investigated gene expression. PO chemical characterization has shown two significant triterpenes, identified as oleanolic acid and hederagenin. The results showed that the PO did not reduce cell viability at concentrations ranging from 31 to 500 mu g/ml. Comet and micronucleus assays revealed the absence of genotoxic effects, and flow cytometry showed no cell cycle or apoptosis disturbance. RT-qPCR indicated that PO up-regulated genes related to metabolism (CYP3A4, CYP1A2, CYP1A1), cell proliferation (mTOR), and oxidative stress (GPX1). The data indicate (AU)

• FAPESP's process: 17/24149-4 - Study of the cytotoxicity, genotoxicity, cell cycle monitoring, induction of apoptosis, and gene expression of Rubus rosaefolius leaves and stems extracts in human cultured cells.
• Grantee: Edson Luis Maistro
• Support Opportunities: Regular Research Grants