| Grant number: | 17/24149-4 |
| Support Opportunities: | Regular Research Grants |
| Start date: | May 01, 2018 |
| End date: | April 30, 2020 |
| Field of knowledge: | Biological Sciences - Genetics - Mutagenesis |
| Principal Investigator: | Edson Luis Maistro |
| Grantee: | Edson Luis Maistro |
| Host Institution: | Faculdade de Filosofia e Ciências (FFC). Universidade Estadual Paulista (UNESP). Campus de Marília. Marília , SP, Brazil |
| City of the host institution: | Marília |
| Associated researchers: | Mário Sérgio Mantovani |
Abstract
The Rubus rosaefolius plant recently have been some of its therapeutic properties confirmed by scientific analysis, among them the analgesic, antimicrobial, antihypertensive, antioxidant, antiproliferative effects in tumor cells, diuretic, gastroprotective and antidepressant. Such confirmation makes this plant of great interest to the pharmaceutical industry. However, before the commercial exploitation of R. rosaefolius as a herbal remedy, it is necessary to carry out tests evaluating the biosafety of the use of this plant by humans, ascertaining if it is free of cellular and genetic toxicity. In view of the above context, this research aims to identify the cytotoxic and genotoxic potential in HepG2/C3A and human leukocytes; and effects on the cell cycle progression, action on the apoptosis induction, of leaf and stem extracts of R. rosaefolius, in HepG2/C3A cells (Human hepatoma). In addition, this study will to investigate the possible mechanism of action of the both extracts, through the analysis of the gene expression of some of the main genes of these biochemical pathways. For this, the first stage of the work will seek to observe effects of cytotoxicity of the extract, by MTT test, trypan blue staining, genotoxicity by comet and micronucleus assays, and on cell cycle progression and induction of apoptosis, by flow cytometry. The second stage of the research will include, through real-time qRT-PCR analysis, the investigation of the relative expression of genes involved in the above-mentioned processes that have had some interesting biological effect after exposure to the extracts. The development of this project will allow the formation of qualified human resources at doctoral level and scientific initiation, as well as the implantation in the laboratory, the molecular techniques involving the analysis of gene expression (qRT-PCR), and flow cytometry, which have the potential to elucidate the Mechanisms of action of the phytotherapeutics (and its constituents) routinely investigated in the Toxicological Genetics Laboratory. (AU)
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