Piper cubeba: applications in different tumorigenic cell lines

• Grant number: 17/02100-3
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2017
• End date: November 30, 2019
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Flávia Cristina Rodrigues Lisoni
• Grantee: Flávia Cristina Rodrigues Lisoni
• Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil
• Associated researchers: Eloiza Helena Tajara da Silva ; Rosangela da Silva de Laurentiz ; Sonia Maria Oliani

Abstract

Carcinomas are malignant neoplasms derived from epithelial cells and represent the most common form of human cancer, it is one of the most common diseases, being the second cause of death worldwide. Cancers of the cervix and head and neck cause many deaths in developing countries. Treatment of these types of tumors includes surgery, radiation therapy and chemotherapy. However, these methods are highly invasive and can cause irrecoverable aesthetic lesions with significant functional impairment. Because of this, some plants have been used in the treatment of cancer and one of them has aroused scientific interest, Piper cubeba, used in the phytotherapeutic treatment of gonorrhea, syphilis, abdominal pain, diarrhea, enteritis, asthma and tumors in general. Due to the importance of the antitumor activity of the lignans extracted from Piper cubeba, including its chemical elements, the present project was proposed whose general objective is to evaluate the potential cytotoxic and genotoxic effect of lignans on neoplastic cells on cell morphology, proliferation and migration, cytotoxicity, genotoxicity, apoptosis and cell necrosis, and gene and protein expression, observing how the herbal medicine acts and how these changes can participate in the tumorigenic process. For this, four tumorigenic cell lines and a normal cell line will be used after treatment with the lignans cubebin, hinoquinin, dihydrocubebin, ethylcubebin and metilcubebin, all extracted from the seeds of Piper cubeba. So we can understand some of the mechanisms and interactions of this herbal medicine in the tumorigenic process. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUSSON-ZANETONI, JULIANA PRADO; GUARALDI MONTEIRO DA SILVA, JULLIENE STEPHANIE; PICAO, THAIS BRAVO; CARDIN, LAILA TONIOL; PRATES, JANESLY; SOUSA, STEFANIE OLIVEIRA; HENRIQUE, TIAGO; OLIANI, SONIA MARIA; TAJARA, ELOIZA HELENA; ANDRADE E SILVA, MARCIO LUIS; et al. Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts. JOURNAL OF PHARMACOLOGICAL SCIENCES, v. 148, n. 1, p. 93-102, JAN 2022. (17/02100-3)

CARDOSO, LUANA PEREIRA; DE SOUSA, STEFANIE OLIVEIRA; GUSSON-ZANETONI, JULIANA PRADO; DE MELO MOREIRA SILVA, LAURA LUCIANA DE MELO MOREIRA; FRIGIERI, BARBARA MARIA; HENRIQUE, TIAGO; TAJARA, ELOIZA HELENA; OLIANI, SONIA MARIA; RODRIGUES-LISONI, FLAVIA CRISTINA. Piperine Reduces Neoplastic Progression in Cervical Cancer Cells by Downregulating the Cyclooxygenase 2 Pathway. PHARMACEUTICALS, v. 16, n. 1, p. 21-pg., 2023-01-01. (17/02100-3, 19/19949-7)