| Full text | |
| Author(s): Show less - |
Semeghini Guastaldi, Fernando Pozzi
;
Matheus, Henrique Rinaldi
;
de Souza Faloni, Ana Paula
;
de Almeida-Filho, Edson
;
Cominotte, Mariana Aline
;
Correa Moretti, Livia Alves
;
Arruda Verzola, Mario Henrique
;
Marcantonio Jr, Elcio
;
de Almeida, Juliano Milanezi
;
Guastaldi, Antonio Carlos
;
Cirelli, Joni Augusto
Total Authors: 11
|
| Document type: | Journal article |
| Source: | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 110, n. 12, p. 19-pg., 2022-07-02. |
| Abstract | |
This study aims to evaluate the potential of a novel biomaterial synthesized from amorphous calcium phosphate (ACP), octacalcium phosphate (OCP), and hydroxyapatite (HA) to repair critical-sized defects (CSD) in rabbit calvaria. In vitro analyses of cell viability, cell proliferation, formation of mineral nodules, and cell differentiation using qPCR were performed for comparing experimental calcium phosphate (ECP), deproteinized bovine bone (DBB), and beta-tricalcium phosphate (beta-TCP). Bilateral CSDs were created in 45 rabbit calvaria. Six groups were evaluated: ECP, ECP + fibrin sealant (ECP + S), coagulum, autogenous bone, DBB, and beta-TCP. Euthanasia was performed at 2, 4, and 8 weeks, followed by micro-computed tomography and histological and immunohistochemical analyses. Results from in vitro analyses revealed similar biocompatibility for all tested materials and a tendency for higher gene expression of some bone markers in the ECP group than in beta-TCP and DBB groups at 7 days. In contrast to that in DBB and beta-TCP groups, ECP displayed growing bone volume over total volume percentage (BV/TV%) with time in vivo. Histological analysis revealed a greater number of giant cells and reduced size of grafted particles in ECP during all periods of analysis. RUNX-2 expression was statistically lower in ECP than DBB at 2 and 4 weeks. Despite no statistical significance, ECP presented the highest absolute values for ALP-expression at 2, 4, and 8 weeks compared with other groups. Together, our findings indicate that a combination of the ACP, OCP, and HA phases into ECP is beneficial and promising for bone regeneration. (AU) | |
| FAPESP's process: | 14/16688-4 - Development and biological evaluation of a new calcium phosphate with and without fibrin sealant: an in vivo study in rabbits and in vitro study |
| Grantee: | Fernando Pozzi Semeghini Guastaldi |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |