Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR gamma

Author(s)

Ferraz Carbonel, Adriana Aparecida ; da Silva, Rafael Andre ; de Souza Ferreira, Luiz Philipe ; Vieira, Renata Ramos ; dos Santos Simoes, Ricardo ; da Silva Sasso, Gisela Rodrigues ; de Jesus Simoes, Manuel ; Soares Junior, Jose Maria ; Azevedo Lima, Patricia Daniele ; Borges, Fernanda Teixeira

• Document type: Journal article
• Source: NUTRIENTS; v. 14, n. 13, p. 11-pg., 2022-07-01.

Abstract

Diabetes associated with post-menopause is related to a worse condition of kidney disease. Taking into consideration that this disorder may be regulated by estrogenic mediators, we evaluated the renal protective effect of isoflavone. We investigated the role of the PPAR gamma in the pathogenesis of the disease. For this study, we used diabetic female rats in a postmenopausal model through ovariectomy. The animals were treated with isoflavone or 17 beta-estradiol. A dosage was administered to bring on blood glycemia, and through immunohistochemistry, we evaluated the immunoreactivity of PPAR gamma in the endometrium and renal tissue. We analyzed the immunoreactivity of renal injury molecule KIM-1 and the collagen and glycogen densities in the kidney. Through bioinformatics analysis, we observed PPAR gamma and COL1A1 gene expression under the influence of different glucose doses. In particular, we observed that isoflavone and 17 beta-estradiol regulate blood glycemia. Renal injury was inhibited by isoflavone, observed by a reduction in KIM-1, along with glycogen accumulation. These benefits of isoflavone may be associated with PPARy overexpression in the kidneys and endometrium of diabetic ovariectomized rats. (AU)

• FAPESP's process: 20/13405-2 - Evaluation of the parameters of protective mechanical ventilation on the renal function of obese animals: renal consequences of prolonged treatment
• Grantee: Fernanda Teixeira Borges
• Support Opportunities: Regular Research Grants