| Full text | |
| Author(s): |
Bras-Costa, Carolina
;
Chaves, Alison Felipe Alencar
;
Cajado-Carvalho, Daniela
;
da Silva Pires, David
;
Andrade-Silva, Debora
;
Serrano, Solange M. T.
Total Authors: 6
|
| Document type: | Journal article |
| Source: | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1870, n. 7, p. 15-pg., 2022-07-01. |
| Abstract | |
Snake venom proteomes have long been investigated to explore a multitude of biologically active components that are used for prey capture and defense, and are involved in the pathological effects observed upon mammalian envenomation. Glycosylation is a major protein post-translational modification in venoms and contributes to the diversification of proteomes. We have shown that Bothrops venoms are markedly defined by their content of glycoproteins, and that most N-glycan structures of eight Bothrops venoms contain sialic acid, while bisected N-acetylglucosamine was identified in Bothrops cotiara venom. To further investigate the mechanisms involved in the generation of different venoms by related snakes, here the glycoproteomes of nine Bothrops venoms (Bothrops atrox, B. cotiara, Bothrops erythromelas, Bothrops fonsecai, B. insularis, Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni and Bothrops neuwiedi) were comparatively analyzed by enrichment with three lectins of different specificities, recognizing bisecting N-acetylglucosamine- and sialic acid-containing glycoproteins, and mass spectrometry. The lectin capture strategy generated venom fractions enriched with several glycoproteins, including metalloprotease, serine protease, and L- amino acid oxidase, in addition to various types of low abundant enzymes. The different contents of lectin-enriched proteins underscore novel aspects of the variability of the glycoprotein subproteomes of Bothrops venoms and point to the role of distinct types of glycan chains in generating different venoms by closely related snake species. (AU) | |
| FAPESP's process: | 15/23017-1 - Analysis of the complexity of venom glycoproteomes of Bothrops genus |
| Grantee: | Carolina Brás Costa |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 17/09929-3 - Bothrops snake venoms: impact of glycosylation on the complexity of proteomes and toxins function |
| Grantee: | Carolina Brás Costa |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 13/13548-4 - Proteomic/Glycoproteomic characterization of the venoms of the Bothrops jararaca complex with emphasis on the N-terminome and N-glycome of toxins |
| Grantee: | Solange Maria de Toledo Serrano |
| Support Opportunities: | Regular Research Grants |