Antiproliferative Effect and Autophagy Inhibition of Dihydropyrimidinone-Cinnamic Acid Hybrids

Author(s)

Barbosa, Flavio Augusto Rocha ; Rode, Michele Patricia ; Santos Canto, Romulo Faria ; Silva, Adny Henrique ; Creczynski-Pasa, Tania Beatriz ; Braga, Antonio Luiz

• Document type: Journal article
• Source: CHEMISTRYSELECT; v. 7, n. 27, p. 8-pg., 2022-07-21.

Abstract

Here we report the design, synthesis and evaluation of dihydropyrimidinones (DHPMs)-cinnamic acids hybrids as potential anti-cancer compounds. The synthetic route was successfully performed and the products obtained in good to excellent yields. These compounds were tested against LNCaP and PC-3 metastatic prostate cancer cells and RWPE-1 prostate cells. The results indicate that hybridization was effective, since the hybrids were much more cytotoxic than DHPMs and cinnamic acids evaluated separately. Compound 3 a was the most potent hybrid against PC-3 cells (IC50 of 15.7 mu M) and LNCaP cells (IC50 of 11.5 mu M) and was further optimized by bioisosterism and molecular simplification strategies leading to three new analogues (5, 7 and 8). Compounds 3 a, 7 and 8 showed an antiproliferative effect against PC-3 cells without inducing cell death nor cell cycle arrest. The mechanism of action of compound 8 was further investigated. The results indicated that compound 8 inhibits autophagic flux, and this effect could be linked to the antiproliferative activity. Moreover, in silico prediction of some molecular properties related to pharmacokinetics showed that all the hybrids meet the criteria for a drug prototype to have a good absorption and permeation for oral administration. These results highlight the potential use of DHPMs-cinnamic acids hybrids as antiproliferative agents for further evaluation of the antitumor activity in vivo. (AU)

• FAPESP's process: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
• Grantee: Arlene Gonçalves Corrêa
• Support Opportunities: Research Grants - Research Centers in Engineering Program