Vanillin crosslinked chitosan films: The states of water and the effect of carriers on curcumin uptake

Author(s)

da Silva, Rafael L. C. G. ; Bernardinelli, Oigres D. ; Frachini, Emilli C. G. ; Ulrich, Henning ; Sabadini, Edvaldo ; Petri, Denise F. S.

• Document type: Journal article
• Source: Carbohydrate Polymers; v. 292, p. 10-pg., 2022-06-14.

Abstract

In this work, chitosan chains were crosslinked with different contents of vanillin (Van), characterized and loaded with curcumin (CUR), a hydrophobic drug. Sodium dodecyl sulfate (SDS), Tween 20 (R) (T20) and beta-cyclodextrin (beta CD) were used as curcumin carriers. Films prepared with Van 20 % yielded gel content of 70 %, swelling degree of similar to 23 g(water)/g, bound water and capillary water, as revealed by Time-Domain Nuclear Magnetic Resonance measurements. Films prepared with higher Van contents showed small swelling degree (< 1.6 g(water)/g) and hydrophobicity, making them inadequate for drug loading. UV-Vis and fluorescence spectroscopic studies indicated that Van 20 % combined with SDS and SDS/beta CD presented the highest CUR uptake (similar to 3.0 mg/g), favored by electrostatic interactions and hydrophobic interactions. CHI and Van 20 % films did not present any cytotoxicity in human neuroblastoma SH-SY5Y cells. At pH 1.0 the films were completely soluble, pointing to their potential application as gastric delivery systems for hydrophobic drugs. Chemical compounds studied in the manuscript: Chitosan, vanillin, curcumin, beta-cyclodextrin, sodium dodecyl sulfate, polyethylene glycol sorbitan monolaurate. (AU)

• FAPESP's process: 20/05632-9 - Nanotopography and nanomechanical properties of nanostructured chitosan scaffolds and of cells adhered on them
• Grantee: Rafael Leonardo Cruz Gomes da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine
• Grantee: Luiz Henrique Catalani
• Support Opportunities: Research Projects - Thematic Grants