| Full text | |
| Author(s): Show less - |
Silva-Vignato, Barbara
;
Cesar, Aline Silva Mello
;
Afonso, Juliana
;
Moreira, Gabriel Costa Monteiro
;
Poleti, Mirele Daiana
;
Petrini, Juliana
;
Garcia, Ingrid Soares
;
Clemente, Luan Gaspar
;
Mourao, Gerson Barreto
;
Regitano, Luciana Correia de Almeida
;
Coutinho, Luiz Lehmann
Total Authors: 11
|
| Document type: | Journal article |
| Source: | FRONTIERS IN GENETICS; v. 13, p. 15-pg., 2022-08-04. |
| Abstract | |
Understanding the architecture of gene expression is fundamental to unravel the molecular mechanisms regulating complex traits in bovine, such as intramuscular fat content (IMF) and backfat thickness (BFT). These traits are economically important for the beef industry since they affect carcass and meat quality. Our main goal was to identify gene expression regulatory polymorphisms within genomic regions (QTL) associated with IMF and BFT in Nellore cattle. For that, we used RNA-Seq data from 193 Nellore steers to perform SNP calling analysis. Then, we combined the RNA-Seq SNP and a high-density SNP panel to obtain a new dataset for further genome-wide association analysis (GWAS), totaling 534,928 SNPs. GWAS was performed using the Bayes B model. Twenty-one relevant QTL were associated with our target traits. The expression quantitative trait loci (eQTL) analysis was performed using Matrix eQTL with the complete SNP dataset and 12,991 genes, revealing a total of 71,033 cis and 36,497 trans-eQTL (FDR < 0.05). Intersecting with QTL for IMF, we found 231 eQTL regulating the expression levels of 117 genes. Within those eQTL, three predicted deleterious SNPs were identified. We also identified 109 eQTL associated with BFT and affecting the expression of 54 genes. This study revealed genomic regions and regulatory SNPs associated with fat deposition in Nellore cattle. We highlight the transcription factors FOXP4, FOXO3, ZSCAN2, and EBF4, involved in lipid metabolism-related pathways. These results helped us to improve our knowledge about the genetic architecture behind important traits in cattle. (AU) | |
| FAPESP's process: | 19/04089-2 - The Nelore hologenome: implications in beef quality and feed efficiency |
| Grantee: | Luciana Correia de Almeida Regitano |
| Support Opportunities: | Research Grants - eScience and Data Science Program - Thematic Grants |