| Full text | |
| Author(s): |
Bronczek, Gabriela Alves
;
Soares, Gabriela Moreira
;
Marmentini, Carine
;
Boschero, Antonio Carlos
;
Costa-Junior, Jose Maria
Total Authors: 5
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 16, p. 17-pg., 2022-08-01. |
| Abstract | |
Resistance training increases insulin secretion and beta cell function in healthy mice. Here, we explored the effects of resistance training on beta cell glucose sensing and survival by using in vitro and in vivo diabetic models. A pancreatic beta cell line (INS-1E), incubated with serum from trained mice, displayed increased insulin secretion, which could be linked with increased expression of glucose transporter 2 (GLUT2) and glucokinase (GCK). When cells were exposed to pro-inflammatory cytokines (in vitro type 1 diabetes), trained serum preserved both insulin secretion and GCK expression, reduced expression of proteins related to apoptotic pathways, and also protected cells from cytokine-induced apoptosis. Using 8-week-old C57BL/6 mice, turned diabetic by multiple low doses of streptozotocin, we observed that resistance training increased muscle mass and fat deposition, reduced fasting and fed glycemia, and improved glucose tolerance. These findings may be explained by the increased fasting and fed insulinemia, along with increased beta cell mass and beta cell number per islet, observed in diabetic-trained mice compared to diabetic sedentary mice. In conclusion, we believe that resistance training stimulates the release of humoral factors which can turn beta cells more resistant to harmful conditions and improve their response to a glucose stimulus. (AU) | |
| FAPESP's process: | 13/07607-8 - OCRC - Obesity and Comorbidities Research Center |
| Grantee: | Licio Augusto Velloso |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 21/04664-7 - Molecular mechanisms involved in pancreatic beta cell dysfunction and death in Diabetes Mellitus: strategies for the inhibition of these processes and restoration of the insular mass |
| Grantee: | Antonio Carlos Boschiero |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/15032-9 - Effect of resistance exercise training upon pancreatic beta-cell mass and function in healthy and diabetic mice |
| Grantee: | Gabriela Alves Bronczek |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/12611-0 - Molecular mechanisms involved in pancreatic beta cell disfunction and dead in diabetes mellitus: strategies for the inhibition of these processes and restoration of the insular mass |
| Grantee: | Antonio Carlos Boschiero |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/17102-9 - Function of myostatin in epigenetic control of pancreatic beta cells proliferation |
| Grantee: | José Maria Costa Júnior |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |