| Full text | |
| Author(s): |
dos Santos, Danilo M.
;
Dias, Luana M.
;
Surur, Amanda K.
;
de Moraes, Daniel A.
;
Pavarina, Ana C.
;
Fontana, Carla R.
;
Correa, Daniel S.
Total Authors: 7
|
| Document type: | Journal article |
| Source: | ACS APPLIED NANO MATERIALS; v. 5, n. 10, p. 12-pg., 2022-09-22. |
| Abstract | |
Oxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO(2)) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO(2 )NPs) and manganese dioxide nanosheets (MnO2 NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2 NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2 NSs were applied as a nanozyme to catalyze the decomposition of H2O2 to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2 NPs and MnO2 NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2 NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2 NP and MnO2 NS were incorporated into the fiber's structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration. (AU) | |
| FAPESP's process: | 17/20973-4 - Coaxial electrospun nanofibers based on chitosan for controlled release of antibiotics and periodontic lesion treatment |
| Grantee: | Danilo Martins dos Santos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 20/16227-8 - Influence of photodynamic therapy combined with antimicrobial peptides on Candida albicans biofilm and 3D cell culture |
| Grantee: | Luana Mendonça Dias Santana |
| Support Opportunities: | Scholarships in Brazil - Doctorate |