Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies

Author(s):Show less -

Capetti, Caio Cesar de Mello ; Pellegrini, Vanessa Oliveira Arnoldi ; Santo, Melissa Cristina Espirito ; Cortez, Anelyse Abreu ; Falvo, Mauricio ; Curvelo, Antonio Aprigio da Silva ; Campos, Eleonora ; Filgueiras, Jefferson Gonsalves ; Guimaraes, Francisco Eduardo Gontijo ; de Azevedo, Eduardo Ribeiro ; Polikarpov, Igor

• Document type: Journal article
• Source: Carbohydrate Polymers; v. 299, p. 12-pg., 2023-01-01.

Abstract

Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via "green" and sustainable XOS production. (AU)

• FAPESP's process: 19/10942-0 - Heterologous expression and characterization of xilanases that belong to GH10 and GH11 families and evaluation of its application in xilan cleavage leading to xylooligossacharides production from lignocelulosic biomass
• Grantee: Caio Cesar de Mello Capetti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation
• Grantee: Igor Polikarpov
• Support Opportunities: Research Projects - Thematic Grants