Lignocellulolytic enzymes from filamentous fungi: identification, purification, ch...
| Grant number: | 19/10942-0 |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| Effective date (Start): | July 01, 2019 |
| Effective date (End): | June 30, 2020 |
| Field of knowledge: | Biological Sciences - Biophysics |
| Principal Investigator: | Igor Polikarpov |
| Grantee: | Caio Cesar de Mello Capetti |
| Home Institution: | Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil |
| Associated research grant: | 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation, AP.TEM |
Abstract The greatest fraction of hemicellulose of plants is represented by xylan, which, after cellulose, is the most abundant natural and renewable polissacharide in nature. Hemicellulases, the enzymes capable of clevating beta 1,4 linkage that keeps together the monomers of xylose, building the main chain of xylan, are divided in many glycoside hydrolase families, the main ones being GH10 and GH11. Enzymatic degradation of xylan may produce monomers of xylose as well as oligossacharides (xylooligossacharides, XOs). XOs are considered to be pre-biotics, as they promote selective growth of beneficial microorganisms that make up the intestinal flora, such as those of the genus Bifidobacterium, responsible for many good effects on human health. In this project, we propose making biochemical and biophysical characterizations of two xylanases, from families GH10 and GH11, as well as its use in xylan degradation and XOS production from lignocellulosic biomass. The biochemical and biophysical properties of the enzymes, and the products obtained will be compared, which is convenient because xylanases with different properties are important to produce xylooligossacharides. This could grant us products with different degrees of polimeriation and ramification. | |