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Heterologous expression and characterization of xilanases that belong to GH10 and GH11 families and evaluation of its application in xilan cleavage leading to xylooligossacharides production from lignocelulosic biomass

Grant number: 19/10942-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2019
Effective date (End): June 30, 2020
Field of knowledge:Biological Sciences - Biophysics
Principal Investigator:Igor Polikarpov
Grantee:Caio Cesar de Mello Capetti
Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation, AP.TEM

Abstract

The greatest fraction of hemicellulose of plants is represented by xylan, which, after cellulose, is the most abundant natural and renewable polissacharide in nature. Hemicellulases, the enzymes capable of clevating beta 1,4 linkage that keeps together the monomers of xylose, building the main chain of xylan, are divided in many glycoside hydrolase families, the main ones being GH10 and GH11. Enzymatic degradation of xylan may produce monomers of xylose as well as oligossacharides (xylooligossacharides, XOs). XOs are considered to be pre-biotics, as they promote selective growth of beneficial microorganisms that make up the intestinal flora, such as those of the genus Bifidobacterium, responsible for many good effects on human health. In this project, we propose making biochemical and biophysical characterizations of two xylanases, from families GH10 and GH11, as well as its use in xylan degradation and XOS production from lignocellulosic biomass. The biochemical and biophysical properties of the enzymes, and the products obtained will be compared, which is convenient because xylanases with different properties are important to produce xylooligossacharides. This could grant us products with different degrees of polimeriation and ramification.