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Development of enzymatic cocktails for modification of xylan and production of derivatives

Grant number: 18/07648-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2019
Effective date (End): September 30, 2022
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Michel Brienzo
Grantee:Danilo Bueno
Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil


The recalcitrance of lignocellulosic biomass has been indicated as a barrier to the technical-economical feasibility in the process of macromolecules extraction and production of high-added value products. Naturally, the lignocellulosic materials are resistents to enzimatic and microbian degradation, which occurs due the organization of its components, where the cellulose is embedded in a matrix composed by hemicellulose and lignin. Therefore, to extract the hemicelullose from sugar cane bagasse without residual lignin is a great challenge. The xylan has several applications, depending on its structure: polymeric, oligomeric, monomeric, with or without branching (xylooligosaccharides, xylose, hydrogels, packaging, artificial skin, etc.). An alternative approach to chemistry pathway is to develop an enzymatic extract capable of: (1) to complete hydrolysis of xylan, containing enzymes that cleave linkages of both the main chain and its pendant groups; (2) cleaving the bonds with the pendant groups, generating a free xylan chain of pendant groups; (3) cleaving different bonds in the main chain generating products with different degree of polymerization; (4) cleaving bonds between the xylan chain and the residual lignin. Thus, this project aims to develop enzymatic extracts (heterologous enzymes) that can: lead to complete hydrolysis ofxylan; to remove pendant groups from the xylan main chain and thereby producing a suitable xylan for film production; to remove the residual lignin from the xylan chain; and to cleave the main chain generating XOS with different degrees of polymerization. The production of these enzymatic extracts will be carried out through the cultivation of several xylanase producing microorganisms, auxiliary enzymes and ligninases. These extracts will be partially purified and concentrated, according to each objective. Therefore, this project can develop robust enzymatic extracts that will lead to the generation of products and raw materials of great industrial interest. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BUENO, DANILO; DE FREITAS, CAROLINE; BRIENZO, MICHEL. Enzymatic Cocktail Formulation for Xylan Hydrolysis into Xylose and Xylooligosaccharides. Molecules, v. 28, n. 2, p. 12-pg., . (18/07648-0, 19/12997-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BUENO, Danilo. Development of enzymatic cocktails for modification of xylan and production of derivatives. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.

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