| Full text | |
| Author(s): |
Thomaz, Fernanda Malanconi
;
Simao, Jussara de Jesus
;
da Silva, Viviane Simoes
;
Forcelini Machado, Meira Maria
;
Oyama, Lila Missae
;
Ribeiro, Eliane Beraldi
;
Cardoso Alonso Vale, Maria Isabel
;
Telles, Monica Marques
Total Authors: 8
|
| Document type: | Journal article |
| Source: | PHARMACEUTICALS; v. 15, n. 10, p. 14-pg., 2022-10-01. |
| Abstract | |
Smaller adipocytes are related to the reversal of metabolic disorders, suggesting that molecules that can act in the adipogenesis pathway are of great interest. The objective of this study was to investigate the effect of Ginkgo biloba extract (GbE) in modulating the differentiation in preadipocytes. 3T3-L1 preadipocytes were differentiated for 7 days into adipocytes without (control group) and with GbE at 1.0 mg/mL. Lipid content and gene expression were analyzed on day 7 (D7) by Oil Red O staining and PCR Array Gene Expression. Western blotting analysis of the key adipogenesis markers was evaluated during the differentiation process at days 3 (D3), 5 (D5), and 7 (D7). GbE increased lipid content and raised the gene expression of the main adipogenesis markers. Key proteins of the differentiation process were modulated by GbE, since C/EBP beta levels were decreased, while C/EBP alpha levels were increased at D7. Regarding the mature adipocytes' markers, GbE enhanced the levels of both FABP4 at D5, and perilipin at D3 and D5. In summary, the present findings showed that GbE modulated the adipogenesis pathway suggesting that the treatment could accelerate the preadipocyte maturation, stimulating the expression of mature adipocyte proteins earlier than expected. (AU) | |
| FAPESP's process: | 14/18929-9 - Investigation of potential mechanisms of action of standardized Ginkgo biloba extract (GbE) involved in the improvement of diet-induced obesity and insulin resistance |
| Grantee: | Monica Marques Telles |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/13618-9 - Modulation of Ezh2 expression, and other H3K27 modifiers, by NF-kB transcription factors in adipose cells |
| Grantee: | Maria Isabel Cardoso Alonso-Vale |
| Support Opportunities: | Regular Research Grants |