Investigation of potential mechanisms of action of standardized Ginkgo biloba extract (GbE) involved in the improvement of diet-induced obesity and insulin resistance

• Grant number: 14/18929-9
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Pharmacology
• Principal Investigator: Monica Marques Telles
• Grantee: Monica Marques Telles
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• City of the host institution: Diadema
• Associated researchers: Cristina Viana Niero ; Eliane Beraldi Ribeiro ; Iracema Senna de Andrade ; Lila Missae Oyama ; Luciana Chagas Caperuto ; Maria Isabel Cardoso Alonso-Vale

Abstract

Obesity is a chronic disease which has been associated to a high incidence of metabolic changes. Taking into consideration the risks of obesity-related disorders, such as diabetes and cardiovascular diseases, the development of an efficient and easy access therapy is highly desirable. Studies from our laboratory have shown that obese mice treated with standardized Ginkgo biloba extract (GbE), one of the most widely used herbal medicines worldwide, improved insulin sensitivity and increased signaling of this hormone in insulin-dependent tissues of obese rats. These data allowed us to suggest the potential use of EGb as a therapy for preventing or treating metabolic complications from obesity. In this context, the present study aims to examine whether the previously observed effects of GbE occur through changes in intestinal microbiota, plasma proteome and both lipid metabolism and proteome of the retroperitoneal fat depot. For this, mice will be fed with high fat diet from 2 to 4-mo-old. Following, animals will receive a daily gavage with GbE for a period of 14 days. After the treatment, rats will be euthanized and we will evaluate the plasma proteome, retroperitoneal fat depot proteome and lipid metabolism, as well as the GUT microbiota. We will also measure the daily food intake, body weight gain, weight of retroperitoneal, epididymal and mesenteric fat depots as well as serum levels of insulin, leptin, adiponectin, IL-6, IL-1², TNF-±, IL-10 and LPS after treatment. (AU)

Scientific publications

(5)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

THOMAZ, FERNANDA MALANCONI; SIMAO, JUSSARA DE JESUS; DA SILVA, VIVIANE SIMOES; FORCELINI MACHADO, MEIRA MARIA; OYAMA, LILA MISSAE; RIBEIRO, ELIANE BERALDI; CARDOSO ALONSO VALE, MARIA ISABEL; TELLES, MONICA MARQUES. Ginkgo biloba Extract Stimulates Adipogenesis in 3T3-L1 Preadipocytes. PHARMACEUTICALS, v. 15, n. 10, p. 14-pg., 2022-10-01. (14/18929-9, 19/13618-9)

HIRATA, BRUNA K. S.; CRUZ, MAYSA M.; DE SA, ROBERTA D. C. C.; FARIAS, TALITA S. M.; MACHADO, MEIRA M. F.; BUENO, ATTAIN A.; ALONSO-VALE, MARIA ISABEL C.; TELLES, MONICA M.. Potential Anti-obesogenic Effects of Ginkgo biloba Observed in Epididymal White Adipose Tissue of Obese Rats. FRONTIERS IN ENDOCRINOLOGY, v. 10, MAY 10 2019. (14/18929-9)

HIRATA, BRUNA K. S.; PEDROSO, AMANDA P.; MACHADO, MEIRA M. F.; NETO, NELSON I. P.; PERESTRELO, BRUNA O.; DE SA, ROBERTA D. C. C.; ALONSO-VALE, MARIA ISABEL C.; NOGUEIRA, FERNANDO N.; OYAMA, LILA M.; RIBEIRO, ELIANE B.; et al. Ginkgo biloba Extract Modulates the Retroperitoneal Fat Depot Proteome and Reduces Oxidative Stress in Diet-Induced Obese Rats. FRONTIERS IN PHARMACOLOGY, v. 10, JUN 14 2019. (14/18929-9)

MACHADO, MEIRA M. F.; ATICO, ESTHER M.; BANIN, RENATA M.; HIRATA, BRUNA K. S.; KEMPE, PAULA R. G.; PEDROSO, AMANDA P.; THOMAZ, FERNANDA M.; OYAMA, LILA M.; RIBEIRO, ELIANE B.; BUENO, ALLAIN A.; et al. Ginkgo biloba extract modulates astrocytic and microglial recruitment in the hippocampus and hypothalamus of menopause-induced ovariectomized rats. Brain Research, v. 1822, p. 14-pg., 2023-10-31. (14/18929-9)

MACHADO, MEIRA MARIA FORCELINI; BANIN, RENATA MANCINI; THOMAZ, FERNANDA MALANCONI; DE ANDRADE, IRACEMA SENNA; BOLDARINE, VALTER TADEU; DE SOUZA FIGUEIREDO, JESSICA; HIRATA, BRUNA KELLY SOUSA; OYAMA, LILA MISSAE; LAGO, JOAO HENRIQUE GHILARDI; RIBEIRO, ELIANE BERALDI; et al. Ginkgo biloba Extract (GbE) Restores Serotonin and Leptin Receptor Levels and Plays an Antioxidative Role in the Hippocampus of Ovariectomized Rats. Molecular Neurobiology, v. 58, n. 6, p. 2692-2703, JUN 2021. (14/18929-9)