Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (H-1-NMR)-Based Analysis

Author(s)

Foratori-Junior, Gerson Aparecido ; Le Guennec, Adrien ; da Silva Fidalgo, Tatiana Kelly ; Cleaver, Leanne ; Rabelo Buzalaf, Marilia Afonso ; Carpenter, Guy Howard ; de Carvalho Sales-Peres, Silvia Helena

• Document type: Journal article
• Source: METABOLITES; v. 12, n. 11, p. 18-pg., 2022-11-01.

Abstract

This study aimed to elucidate the metabolomic signature associated with obesity and periodontitis during pregnancy in plasma and saliva biofluids. Ninety-eight pregnant women were divided into: with obesity and periodontitis (OP = 20), with obesity but without periodontitis (OWP = 27), with normal BMI but with periodontitis (NP = 21), with normal BMI and without periodontitis (NWP = 30). Saliva and plasma were analyzed by H-1-NMR for metabolites identification. Partial Least Squares-Discriminant Analysis (PLS-DA), Sparse PLS-DA (sPLS-DA), and Variable Importance of Projection (VIP) were performed. ANOVA and Pearson's correlation were applied (p < 0.05). Plasmatic analysis indicated the levels of glucose (p = 0.041) and phenylalanine (p = 0.015) were positively correlated with periodontal parameters and BMI, respectively. In saliva, periodontitis was mainly associated with high levels of acetic acid (p = 0.024), isovaleric acid, butyric acid, leucine, valine, isoleucine, and propionic acid (p < 0.001). High salivary concentrations of glycine (p = 0.015), succinic acid (p = 0.015), and lactate (p = 0.026) were associated with obesity. Saliva demonstrated a more elucidative difference than plasma, indicating the glucose-alanine cycle, alanine metabolism, valine, leucine and isoleucine degradation, glutamate metabolism, and Warburg effect as the main metabolic pathways. (AU)

• FAPESP's process: 19/26845-3 - Systemic and oral evaluations and metabolomic profiling of the saliva and plasma by quantitative proton nuclear magnetic resonance (1H-NMR) in obese and eutrophic pregnant women
• Grantee: Gerson Aparecido Foratori Junior
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/20626-5 - Evaluation of the proteins differentially expressed in saliva associated with periodontitis during the 3rd trimester of pregnancy in obese and eutrophic patients
• Grantee: Gerson Aparecido Foratori Junior
• Support Opportunities: Scholarships in Brazil - Doctorate